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Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations
Courtesy of American Society for Microbiology (ASM)
We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.
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