Candida albicans is usually a harmless human commensal. Because inflammatory responses are not normally induced by colonization, antimicrobial peptides are likely integral to first-line host defense against invasive candidiasis. Thus, C. albicans must have mechanisms to tolerate or circumvent molecular effectors of innate immunity and thereby colonize human tissues. Prior studies demonstrated that an antimicrobial peptide-resistant strain of C. albicans, 36082R, is hypervirulent in animal models versus its susceptible counterpart (36082S). The current study aimed to identify a genetic basis for antimicrobial peptide resistance in C. albicans. Screening of a C. albicans genomic library identified SSD1 as capable of conferring peptide resistance to a susceptible surrogate, Saccharomyces cerevisiae. Sequencing confirmed that the predicted translation products of 36082S and 36082R SSD1 genes were identical. However, Northern analyses corroborated that SSD1 is expressed at higher levels in 36082R than in 36082S. In isogenic backgrounds, ssd1/ssd1 null mutants were significantly more susceptible to antimicrobial peptides than parental strains but had equivalent susceptibilities to nonpeptide stressors. Moreover, SSD1 complementation of ssd1/ssd1 mutants restored parental antimicrobial peptide resistance phenotypes, and overexpression of SSD1 conferred enhanced peptide resistance. Consistent with these in vitro findings, ssd1 null mutants were significantly less virulent in a murine model of disseminated candidiasis than were their parental or complemented strains. Collectively, these results indicate that SSD1 is integral to C. albicans resistance to host defense peptides, a phenotype that appears to enhance the virulence of this organism in vivo.
- American Society for Microbiology
- Ssd1 is integral to host defense peptide resistance in candida ...
New paper about “Enhanced corrosion resistance of metal surfaces by film forming amines”
Kurita, SABIC, CR Competence and Malmö University put their heads together for FFA research. We encourage you to read this paper about “Enhanced corrosion resistance of metal surfaces by film forming amines: A comparative study between cyclohexanamine and 2-(diethylamino) ethanolbased formulations”.
Not All Stainless Steel Resists Corrosion
For Municipalities and Municipal Utilities Authorities struggling to deal with corrosion issues, knowing where to turn when faced with these challenges can be daunting. Logan Township Municipal Utilities Authority, of NJ was faced with such problems when it was discovered that a five year old stainless steel headworks tank at their wastewater treatment facility was experiencing extreme Microbiological Induced Corrosion (MIC) at the waterline with corrosion severe enough to open holes through the tank wall....
Using Resistivity for Basalt Site Characterization - Case Study
Purpose A mining company in Turkey hoped to determine existing and new alternative basalt zones at an existing basalt mining site by using Electrical Resistivity Imaging (ERI). Process Our client, Ali Karan, used 84 electrodes spaced at 10 meters and two lines 5 meters apart inside the study area. The dipole-gradient array was used to collect the 2D electrical resistivity imaging data set. Results Resistivity imaging mapped the vertical and lateral limits of basalt zones successfully. The ground truth was...
2D Resistivity Imaging for Locating Concrete Pipe - Case Study
Background There are 8 wells that suply water to the communnity through about 2m diamter concrete aqueduct. Purpose The main subject of the field tes was to determine the geotechnical site characterization of the area to correlate the results obtained through the 2D resistivity imaging and the infromation of the concrete aqueduct. Process The determination of site characterization was performed by AGI SuperSting Wi-Fi R8/IP/SP instrument with 42 electrodes and 3 meters electrode spacing with a mixed array...
Triclosan resistant bacteria in sewage effluent and cross-resistance to antibiotics
The purpose of this study was to identify triclosan tolerant heterotrophic plate count (HPC) bacteria from sewage effluent and to determine cross-resistance to antibiotics. R2 agar supplemented with triclosan was utilised to isolate triclosan resistant bacteria and 16S rRNA gene sequencing was conducted to identify the isolates. Minimum inhibitory concentrations (MICs) of organisms were determined at selected concentrations of triclosan and cross-resistance to various antibiotics was performed. High-performance...