Nonnutritive sweeteners are widely used in foods and beverages. Since some studies raised questions about long-term safety of these compounds, their concentration in food products is regulated. Among the artificial sweeteners used worldwide Saccharin and Cyclamate cause most controversy and their use is restricted in many countries, including the US. Liquid chromatography is a method of choice for artificial sweeteners but Cyclamate analysis is complicated by the fact that this compound does not exhibit noticeable UV absorbance.
This method allows for simultaneous analysis of Acesulfame, Saccharin and Cyclamate by LC with post-column derivatization followed by fluorescence detection. Since the sweeteners are commonly used in combination, a single method of analysis is preferred.