Survival of E. coli O157:H12 in creek sediments after inoculation and re–inoculation
This work tested the hypothesis that E. coli survival after streambed sediment inoculation does not differ significantly from survival in the same sediment after re–inoculation. The E. coli O157:H12 survived in two sediments with markedly different particle size distributions and organic matter contents in one–month–long experiments in flow–through chambers at 14°C and 23°C. The inactivation rates after re–inoculation were smaller than after inoculation; the differences were significant (P < 0.1) in two of four experiments. This study suggests that E. coli O157:H12 was able to adapt to the sediment environment so that its survival after re–inoculation improved.
Keywords: Escherichia coli, E coli, sediment inoculation, survival, microcosm, inactivation rate, stream beds, particle size, organic matter, re–inoculation