Inderscience Publishers

Survival of E. coli O157:H12 in creek sediments after inoculation and re–inoculation

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This work tested the hypothesis that E. coli survival after streambed sediment inoculation does not differ significantly from survival in the same sediment after re–inoculation. The E. coli O157:H12 survived in two sediments with markedly different particle size distributions and organic matter contents in one–month–long experiments in flow–through chambers at 14°C and 23°C. The inactivation rates after re–inoculation were smaller than after inoculation; the differences were significant (P < 0.1) in two of four experiments. This study suggests that E. coli O157:H12 was able to adapt to the sediment environment so that its survival after re–inoculation improved.

Keywords: Escherichia coli, E coli, sediment inoculation, survival, microcosm, inactivation rate, stream beds, particle size, organic matter, re–inoculation

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