John Wiley & Sons, Ltd.

The stress response of Pseudomonas species to silver nanoparticles at molecular level

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In recent years silver nanoparticles (AgNPs) have been shown to possess broad antibacterial activity. The present study investigated the cytotoxicity of AgNPs on a common soil bacterium Pseudomonas sp. Further the molecular mechanism involved in its stress response to AgNPs was studied. The minimum inhibitory concentration (MIC) of AgNPs was found to be 0.2 mg/L. At sublethal concentration of 0.1 mg/L AgNPs, the protein expression profile of Pseudomonas showed over‐expression of stress proteins such as ribosomal proteins S2 and L9, alkyl hydroperoxides reductase thiol specific antioxidant (AhpC/TSA) family protein, and ketohydroxyglutarate aldolase (KHGA). The upregulation of these proteins was further confirmed by qPCR. The results showed increased expression of ribosomal protein S2, KHGA, AhpC/TSA and ribosomal L9 by 1.09, 3.41, 1.52 and 1.56 fold, respectively (p < 0.05) upon AgNP exposure as compared to control. The study clearly demonstrates that AgNPs are toxic to the soil bacteria and induce oxidative and metabolic stress in them. Environ Toxicol Chem © 2014 SETAC

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