Traitü RUR HS test kit part # 7000011 - application for bulk afalfa seed testing

0

Courtesy of

Intended Use

The intended use of this application is the qualitative (yes/no) determination of the CP4 EPSPS protein in alfalfa bulk seed
samples. The Traitü RUR HS test strip has been validated for a detection limit of one (1) Roundup Ready® alfalfa seed in 600
conventional alfalfa seeds in 5 minutes.

Application Description

The Traitü RUR HS Test Strip detects the CP4 EPSPS protein expressed by a gene derived from Agrobacterium sp. strain CP4.
This gene has been incorporated into herbicide-resistant crops in the Roundup Ready® brands (RUR) from Monsanto and other
companies. The lateral flow test strip has been optimized to detect the CP4 EPSPS protein expressed in the bulk seed samples.
The lateral flow strips in this package are sufficient to detect the presence or absence of the CP4 EPSPS protein in up to 100 bulk seed samples. The Traitü RUR HS test strip can provide a qualitative, threshold determination of the CP4 EPSPS protein in
alfalfa samples consisting of up to 600 seeds.

Principle of the Test

The assay uses a double antibody sandwich format. Antibodies specific to the CP4 EPSPS protein are coupled to a color reagent
and incorporated into the lateral flow strip. When the lateral flow strip is placed in a small amount of an extract from plant tissue
that contains CP4 EPSPS protein, binding occurs between the coupled antibody and the protein. A sandwich is formed with some, but not all the antibody that is coupled to the color reagent. The membrane contains two capture zones, one captures the bound CP4 EPSPS protein and the other captures color reagent. These capture zones display a reddish color when the sandwich and/or unreacted colored reagents are captured in the specific zones on the membrane. The presence of only one line (control line) on the membrane indicates a negative sample and the presence of two lines indicates a positive sample.

Customer comments

No comments were found for Traitü RUR HS test kit part # 7000011 - application for bulk afalfa seed testing. Be the first to comment!