John Wiley & Sons, Ltd.

Transcriptional profiling of the soil invertebrate (Folsomia candida) in pentachlorophenol contaminated soil

0
Pentachlorophenol (PCP) as a widely used pesticide is also considered to be an endocrine disruptor. Molecular effects of chemicals with endocrine disrupting potential on soil invertebrates are largely unknown. In this study we explored the transcriptional expression changes of collembola (Folsomia candida) in response to PCP contamination. A total of 92 genes were significantly differentially expressed at all exposure times and the majority of them were found to be down‐regulated. In addition to the transcripts encoding cytochrome P450s and transferase enzymes, chitin‐binding protein was also identified in the list of common differentially genes. Analyses of Gene Ontology (GO) annotation and enrichment revealed that cell cycle related transcripts were significantly induced by PCP, indicating that it can stimulate the cell proliferation in springtail as reported in human breast cancer cells. We also observed enrichment of functional terms related to steroid receptor and particularly twenty significant differential expressed genes involved in Chitin metabolism in response to PCP exposure. Combined with the confirmation by qPCR, it appears that the adverse effects on reproduction of springtails after exposure to PCP can be attributed to a chemical‐induced delay in the molting cycle and molting associated genes may serve as possible biomarkers for assessing toxicological effects. This article is protected by copyright. All rights reserved

Customer comments

No comments were found for Transcriptional profiling of the soil invertebrate (Folsomia candida) in pentachlorophenol contaminated soil. Be the first to comment!