Homogenous preparations of XenB of Pseudomonas putida, pentaerythritol tetranitrate reductase of Enterobacter cloacae, and N-ethylmaleimide reductase of Escherichia coli, all type II hydride transferases of the Old Yellow Enzyme family of flavoproteins, are shown to reduce the polynitroaromatic compound 2,4,6-trinitrotoluene (TNT). The reduction of this compound yields hydroxylaminodinitrotoluenes and Meisenheimer dihydride complexes, which, upon condensation, yield stoichiometric amounts of nitrite and diarylamines, implying that type II hydride transferases are responsible for TNT denitration, a process with important environmental implications for TNT remediation.
- American Society for Microbiology
- Type ii hydride transferases from different microorganisms yield ...
Pentachlorophenol degradation by Pseudomonas fluorescens
Fluorescent Pseudomonads strains were considered as plant growth promoting bacteria. They exhibited antagonistic activities against phytopathogens and showed bio-fertilizing properties. The strain Pseudomonas fluorescens PsWw128, isolated from wastewater, can use the pentachlorophenol (PCP) as the sole source of carbon and energy. High-performance liquid chromatography (HPLC) and spectrophotometric methods were used to follow the PCP degradation and biomass PsWw128 formation. However, the removal efficiency of...
Identification of the bacterial population in manganese removal filters
The aim of this study was to identify bacteria present in ripened manganese removal filters for drinking water production. The bacterial population was identified with ‘next generation’ DNA sequencing, and specific bacteria were quantified with quantitative polymerase chain reaction (qPCR) and characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. The ‘next generation’ DNA sequencing analysis showed a bacteria population shift from the iron oxidizing...
EPA Proposes Two Test Methods and Guidance for Evaluating Antimicrobial Pesticides
The U.S. Environmental Protection Agency (EPA) has recently announced the availability of two proposed test methods and associated testing guidance for evaluating antimicrobial pesticides against two biofilm bacteria, Pseudomonas aeruginosa and Staphylococcus aureus, for comments. EPA states that registrants of antimicrobial products with public health claims are “required to submit efficacy data to EPA in support of the product’s registration” under the Federal Insecticide, Fungicide, and...
Phenanthrene removal from liquid medium with emphasis on production of biosurfactant
In the current study, there are some considerable issues which focused on: (1) the production of biosurfactant, (2) its correlation with the initial bacterial inoculum in the liquid phase and (3) its effect on polycyclic hydrocarbon (PAH) bioremediation performance. Therefore, two strains Pseudomonas facilis and Pseudomonas spp. were able to form a large clear zone diameter on the oil surface. Phenanthrene (PHE) was also utilized as a sole substrate. Furthermore, biosurfactant production (BP) was detected by...
Survival of antibacterial resistance microbes in hospital-generated recycled wastewater
Hospital wastewater has the potential to be a threat to the hospital environment as it can contain pathogenic bacteria that may facilitate the resistant nature of organisms within effluent or water treatment plants. The recycling of hospital wastewater should have good quality. This study was carried out to highlight the incidence of antibiotic resistant bacteria in hospital-generated recycled water. This study was conducted in a tertiary care teaching hospital during June 2013–June 2014. One hundred and forty...