Liposomes works as carriers for active agents. Ultrasound is an effective tool to prepare and form the liposomes for the entrapment of active agents. Before encapsulation, the liposomes tend to form clusters due to the surface charge-charge interaction of phospholipid polar heads (Mickova et al. 2008), furthermore they have to be opened. By way of example, Zhu et al. (2003) describe the encapsulation of biotin powder in liposomes by ultrasonication. As the biotin powder was added into the vesicle suspension solution, the solution has been sonicated for approx. 1 hour. After this treatment, biotin was entrapped in the liposomes.