John Wiley & Sons, Ltd.

Use of an exposure chamber to maintain aqueous‐phase nanoparticle dispersions for improved toxicity testing in fish

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A novel chamber for maintaining aqueous phase dispersions of nanoparticles (NPs) to enable improved toxicity testing in larval zebrafish was developed. Aqueous concentrations were within 80% of initial NP concentrations, and 96‐h LC50 values were highly reproducible (coefficient of variation <0.16, n=3 tests). Significantly lower toxicity for each NP tested (Ag‐, Cu‐, TiO2‐NPs) in static beakers suggested that traditional acute toxicity tests may underestimate aqueous phase toxicity of NPs. This article is protected by copyright. All rights reserved

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