The aim of the study was to carry out water quality assessment and to identify sources responsible for deterioration of quality in the Malad creek, Mumbai, India. Creek receives sewage and wastewater from various drains and partially treated effluent from Malad and Versova treatment facilities. To assess the water quality, sampling locations were identified in the creek based on discharges of wastewater and sewage. Identified locations were traced in physical space by a global positioning system. Samples were collected during low and high tides and analyzed for physical, chemical and bacteriological parameters such as pH, Turbidity, DO, BOD, NH3-N, PO4 and FC and compared with SW-II Standards. Parameters were also analyzed statistically and correlated to determine the relationship amongst the parameters using SPSS software. The idea was to determine the probable causes contributing to the pollution in the creek. Various options were suggested for improvement in the creek quality based on water quality assessment.