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The BCI laboratory is equipped to perform anaerobic microbiological testing, preparing microcosms from site soil and groundwater in an anaerobic glove box to document microbial processes such as: PCE and TCE dechlorination to ethene, cis-DCE and vinyl chloride dechlorination to ethene, 1,1,1-TCA dechlorination to chloroethane or ethane, 1,2-DCA dechlorination to chloroethane or ethane, Tri- and dichlorobenzenes to monochlorobenzene or benzene,...
Aerobic microcosm tests are carried out to determine the potential for co-metabolic and non-cometabolic oxidation of solvents and petroleum: co-metabolic oxidation of TCE, DCE, VC, chloroethane, mono-chlorobenzene, co-metabolic oxidation of low concentrations of VC (e.g. 2 ug/L) using aerobic ethenotrophic bacateria, aerobic degradation of tri, di, and mono-chlorobenzenes, biodegradation of 1,2-dichloroethane as a carbon and energy source,...
PCE and TCE contaminants in ground water can be biodegraded by naturally occurring anaerobic bacteria by a process known as reductive dechlorination. This occurs when anaerobic bacteria take electrons from small organic compounds (the 'electron donors') and produce H2. The dechlorinating bacteria use the electrons in the H2 to replace a chlorine atom in TCE/PCE.
BCI uses a genetic method (Polymerase Chain Reaction) for detecting the presence of the unique PCE degrading bacterium, Dehalococcoides ethenogenes, in groundwater. Under the right geobiochemical conditions, it can transform not only PCE but its metabolic products TCE, cis-DCE or vinyl chloride to ethene. BCI’s PCR-based identification has been expanded to include Dehalobacter species involved in the reductive dechlorination of...
BCI is able to provide high-rate, PCE and TCE dechlorinating bacteria able to convert TCE completely to ethene, for potential inoculation of site soil and groundwater. Dechlorinating bacteria can be cultured from your site or, bacteria can be selected from BCI's culture collection, and provided in sufficient volume to inoculate an on-site bioreactor, or for direct injection into site wells.