6 services found

Services

  • cDNA Library Construction Services

    cDNA Library Construction Services

    cDNA libraries identify all expressed genes in a tissue. Gene expression, also called protein expression or often simply expression is the process by which a gene's DNA sequence is converted into the structures and functions of a cell. Gene expression is a multi-step process that begins with DNA, which genes are made of, into messenger RNA. It is then followed by post transcriptional modification and translation into a gene product, followed by...

  • Suppressive Subtractive Hybridization (SSH) Library Construction Services

    Suppressive Subtractive Hybridization (SSH) Library Construction Services

    Suppression subtractive hybridization (SSH) is one of the most powerful and popular PCR-based methods for isolating differentially expressed transcripts. Using a small quantity of either total RNA or poly A+ mRNA (2.5 ug) from each of two populations, the SSH procedure simultaneously subtracts and partially normalizes the abundance of target cDNAs in the subtracted population. Two subtraction libraries, forward (“experimental”) and reverse...

  • Real Time PCR

    Real Time PCR

    Real-time PCR is an extremely sensitive and reproducible method to measure levels of gene expression in an organism. Indirectly, the expression of particular genes may be assessed with microarray technology, which can provide a rough measure of the cellular concentration of different messenger RNAs; often thousands at a time. While the name of this type of assessment is actually a misnomer, it is often referred to as expression profiling. The...

  • Custom Gene Array Construction Services

    Custom Gene Array Construction Services

    Gene sequences spotted onto a solid support matrix are called gene arrays (also referred to as microarrays, macroarrays, gene chips, or DNA arrays). An array can consist of hundreds to thousands of cDNAs or oligonucleotide gene sequences spotted in an organized pattern. The arrays can be probed with mRNA obtained from treated tissues or cells in order to obtain a semi-quantitative measure of expression for each of the genes present on the array. Thus,...

  • Gene Sequencing and Annotation

    Gene Sequencing and Annotation

    Sequencing Only Let EcoArray submit your clones to our sequencing facility. Clones are selected and grown in LB broth with glycerol and ampicillin. RCAs are sequenced in the MegaBace sequencer. Sequences will be quality trimmed (prd files required). Vector sequences will also be identified and removed. Additional sequences (i.e., adapters used for subtractive hybridizations) can also be trimmed as long as the sequence information is provided....

  • Statistical Analysis of Arrays

    Statistical Analysis of Arrays

    Each array package comes with the CD required to extract the feature information.  However, the CD does not explain what to do with the data after you have it in a database file.  EcoArray can do the analysis for you.  The microarrays can be any species (e.g., human, rat, mouse) and can be most platforms, including Agilent, Affymetrix and Illumina.