5 products found
Kinesis Inc - Cole-Parmer Instrument Company Products
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Glass Columns
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OmniSep - Cross-Reference to GE Media
OmniSep is designed to be commercially equivalent to leading soft gel media such as Sepharose or Sephadex. To find the right OmniSep media to replace your current GE media, use the cross-reference chart below: GE Media Cross Reference Chart Omnisep IEX Media GE Equivalent A-Q Dash Q Sepharose Fast flow (strong anion) A-DEAE Dash DEAE ...
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Omnifit Columns - Nitrate Water Analysis
Nitrates are essential plant nutrients, but in excess amounts they can cause significant water quality problems. One of these is to accelerate eutrophication where the response of the ecosystem to raised nitrates or phosphates is a “bloom” caused by the great increase of phytoplankton or cyanobacteria. Excess nitrates can also cause dramatic increases in aquatic plant growth and changes in the types of plants and animals that live in the ...
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OmniSep - Pre-Packed Columns for SEC and Desalting
Size exclusion chromatography (SEC) also known as gel filtration (GF) separates molecules on the basis of size. The cross-linked agarose or dextran resin used in SEC is made up of porous beads. As molecules pass through the SEC column, large molecules pass through between the beads, eluting first, while smaller molecules enter the pores and are delayed. By altering the column length and / or flow rate, elution can be refined. The range of molecular ...
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OmniSep - Pre-Packed Hydrophobic Interaction Columns
HIC separates proteins on the basis of hydrophobicity. Hydrophobic groups on the surface of the protein bind reversibly to a hydrophobic ligand on the surface of the chromatography medium. Binding is usually enhanced by high ionic strength buffers such as 1-2M ammonium sulphate or 3M NaCl, making HIC a natural successor to protein precipitation or IEX. Elution is performed by decreasing the salt concentration, stepwise or using a ...
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OmniSep - Pre-Packed Ni2+ Affinity Columns
Affinity chromatography separates proteins by means of a reversible interaction between the target protein and a binding substance (ligand). In immobilized metal ion affinity chromatography (IMAC), histidine-tagged recombinant proteins bind to metal ions, such as nickel. Affinity chromatography offers high selectivity and high resolution, with the result that high purity can often be achieved in one step. The technique also has a concentrating effect, ...