Separation Methods Technologies, Inc. (SMT)

Separation Methods Technologies, Incorporated (SMT, Inc.) is a surface chemistry research corporation. Our goal is to provide chromatographers with outstanding column packing materials and specialty columns for various separation chemistries ranging from analytical to process scale. Our primary focus is on the creation of well organized functional molecules on substrate surfaces for various functions, including chromatographic applications and materials engineering. SMT Inc. was founded in 1993. The company has its corporate headquarters in Newark, DE, with a worldwide distribution network.

Company details

31 Blue Hen Drive , Newark , Delaware 19713 USA

Locations Served

Our Distributors

Business Type:
Industry Type:
Monitoring and Testing - Laboratory Equipment
Market Focus:
Globally (various continents)
Year Founded:


SMT utilizes proprietary bonding technologies that result in bonded phase coverages that approach 100%. SMT’s methods of bonding allow the density of the functional ligands to be controlled with appropriate spacer molecules, a novel procedure that ensures Total Coverage™ and highly cross-linked polysiloxane under layer structure. The results are bonded phases that are well protected and that show unprecedented resistance to both acid and base hydrolysis. SAM technology provides you with widest range of column retention selectivities and performance benefits.
Our Goal
SMT is a surface chemistry research corporation with a goal of providing chromatographers with the highest quality polymeric, monomeric, specialty column, and bulk packing packing materials for various separation chemistries including pharmaceutical and environmental applications.

Our Business Approach
Innovation: For twenty years, SMT has repeatedly led the industry to new standards of measuring performance expected of UHPLC, HPLC and LPLC columns as well as the column packing materials. Our Total Coverage™ bonding scheme has forced many competitors to come up with all kinds of new products that can only mimic. This is exactly why experts in chromatography turn to SMT for their toughest separation problems.

Reproducibility: We are Original Equipment Manufacturer (OEM), and as such, have a complete control over quality and ensuring reproducibility of your chromatography. As an OEM, we utilize proven procedures at every stage of manufacture to guarantee consistency in our silica purity, pore size, particle size, as well as chemical bonding.

Reliability: SMT’s policy has been to never change any product that has an existing customer base; however, when improvements are made, an entirely new SMT product line is introduced. Thus a method developed on any of our products can be retained for very long time.

Product Range: We have introduced over 100 stationary phases that have resulted in over 1,000 products for HPLC, MPLC and LPLC. We offer you products that will match or exceed your expectation of high performance with virtually any HPLC application, from small molecules to large proteins, at microbore separation or production scale.

Technical Support: Our technical support representatives bring years of experience directly to your laboratory, significantly reducing cost of method developments.

Worldwide Capability: SMT has a worldwide distribution network with products available all over United States, Canada, France, Germany, England, Asia, Africa and other locations. SMT delivers the same high quality products throughout the world.


Self-Assembled Monolayers (SAM) are supramolecular organizates resembling, in some respects, the well-known Langmuir-Blodgett (LB) built-up films while displaying other distinct and rather Special features. Much of the interest in SAM stems from their potential in wide range of scientific and technological applications. The first application of SAM in chromatographic separation science was developed at the University of Delaware by Fatunmbi and Wirth. The bonding technique allows ordered monolayers of functional molecules to be chemically immobilized on solid substrates, such as, silica and alumina.

The technique of bonding was termed “horizontal polymerization” due to the fact that there is significant Si-O-Si bridging parallel to the silica substrate. This is achieved by reaction of trifunctional silanizing agent with the silica substrate under anhydrous condition, except for a monolayer of water on silica. This contrasts with conventional polymerization of trifunctional silanes, referred to as “vertical polymerization,” where water is deliberately added to polymerize the reagents before attachment to the surface. The key structural difference here is that horizontal polymerization provides much higher ligand density at the silica surface boundary.

The bonding chemistry utilized for chromatographic applications results in a monolayer coverage with typical thickness in the range of 6-22 Å, depending on the length of the attached ligand. For example, when a C18 ligand is attached, a film thickness of about 22 Å is obtained. Thickness in this range ensures bonding of the ligand both in and around the substrate material without blocking of the pores. Results from the relaxation time measurements, using solid state nuclear magnetic resonance spectroscopy (NMR), showed that the spacer molecules are evenly dispersed within the functional ligand and are not clumped together.

SMT columns for reversed-phase chromatography include SMT-C18, SMT-C8, SMT-Elite C18, SMT-Elite C8, SMT-ODL, SMT-OL, SMT-MEB (including C1, C2, and C4), SMT-C30, SMT-C6, SMT-C12, SMT-C3, SMT-C5, SMT-Phenyl, SMT-ODAQ, SMT-Urea, SMT-ChiralSep, SMT-CIB and other mixed phases.

SMT offers many other columns for ion exchange chromatography including SMT-SAX, SMT-WAX, SMT-SCX, SMT-WCX, SMT-DEAE, SMT-MetalSep. Unlike polymer-based counterparts, these columns are silica-based and are mechanically stable at high chromatographic pressures.

SMT offers packing materials for large-scale purification and Solid Phase Extraction (SPE) processes. Essentially all SMT analytical column packing materials are offered in large particle sizes at significantly reduced prices for process scale column packings and low pressure bulk purifications . Please refer to our bulk packing materials for these products.