sampling container Articles
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Detection of Clostridium botulinum type C in an aquatic area a year after an outbreak of botulism in waterfowl using conventional and molecular methods
A year after an outbreak of botulism in waterfowl, the presence of the causative agent of the disease, Clostridium botulinum type C, was investigated, using bioassay and polymerase chain reaction (PCR). Eighty two mud samples collected at different locations in the Skocjanski zatok nature reserve were investigated. Mouse bioassay revealed that 38 (46.4%) samples contained C. botulinum type C ...
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Detection of Bacillus anthracis spores in postal and environmental samples in cases of suspected bioterrorism
From October 2001 to the end of 2004, a series of postal samples containing material suspected of containing Bacillus anthracis spores were examined. Environmental samples from dwelling places of some addressees and laboratory areas, including equipment, were also examined. Classical culture methods for isolation and identification were slightly modified and conformed to environmental samples. ...
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Exposure time–dependent effects on the relative potencies and additivity of PAHs in the Ah receptor‐based H4IIE‐luc bioassay
In the present study, relative potency factors (REPs) of 16 individual polycyclic aromatic hydrocarbons (PAHs) were investigated using the H4IIE‐luc bioassay. Exposure time‐dependent effects on the REPs were examined using 24, 48, and 72 h of exposure. Seven different mixtures of PAHs were tested for additivity at an exposure time of 24 h. Three of the PAH mixtures were also studied at 48 and ...
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Novel carotenoid-based biosensor for simple visual detection of arsenite: characterization and preliminary evaluation for environmental application
A novel whole-cell arsenite biosensor was developed using the photosynthetic bacterium Rhodopseudomonas palustris no. 7 and characterized. A sensor plasmid containing the operator-promoter region of the ars operon and arsR gene from Escherichia coli and the crtI gene from R. palustris no. 7 was introduced into a blue-green mutant with crtI deleted, R. palustris no. 711. The biosensor ...
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Environmental characterization of surface runoff from three highway sites in Southern Ontario, Canada: 2. Toxicology
Highway runoff is a significant source of contaminants entering many freshwater systems. A battery of bioassays was used to assess the degree of runoff toxicity. The toxicity results are described in the spatial and temporal context and are linked to the runoff chemistry. Runoff samples from three sites, representing different classes of highways (high, intermediate and low traffic intensity), ...
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Evaluation of quantitative polymerase chain reaction assays targeting
Mycobacterium avium ,M. intracellulare , andM. avium subspeciesparatuberculosis in drinking water biofilmsMycobacterium avium (MA), Mycobacterium intracellulare (MI), and Mycobacterium avium subsp. paratuberculosis (MAP) are difficult to culture due to their slow growing nature. A quantitative polymerase chain reaction (qPCR) method for the rapid detection of MA, MI, and MAP can be used to provide data supporting drinking water biofilms as potential sources of human exposure. The aim of this study ...
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Overview of Brucella Abortus
Brucellosis is a zoonotic infectious disease caused by Brucella, and it is one of the most widespread zoonotic diseases in the world, which brings great losses to the animal husbandry industry. Here, we will outline several aspects about the pathogen, living environment, identification, and diagnostic procedures of Brucella, hoping to popularize knowledge about brucellosis. Characteristics of ...
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Guide to isothermal titration calorimetry
What is isothermal titration calorimetry? Isothermal titration calorimetry (ITC) is an experimental method used to measure the heat released or consumed in bimolecular chemical reactions. Chemical reactions can be exothermic or heat-consuming, depending on the relative energy stability of the reactants. Isothermal titration calorimetry can be used to quantify the magnitude of the heat change ...
By Profacgen
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How Oxygen Sensing Technology Can Deliver Faster Test Results For Food And Beverage Microbiology
Oculer Ltd, Ballina, Ireland1 and School of Chemistry and Chemical Engineering, Queens University, Belfast, UK2 Why use O2 sensors? O2 sensors are widely used in many areas of biology and they have been applied for monitoring the activity and growth of bacteria in the environment. There are a small number of reports of their use for the specific detection of microbial growth in the laboratory. A ...
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Evaluation of QIAamp DNA mini kit for removing of inhibitors in detection of Cryptosporidium parvum Oocysts in water samples by a nested– PCR assay
In recent years, there has been a dramatic increase in the occurrence of waterborne disease outbreaks caused by the Cryptosporidium parvum, and presence of this protozoan parasite in drinking water is a significant health problem faced by the water industry. A new strategy for detection of Cryptosporidium oocysts in water samples is PCR– based techniques. In this study a nested– PCR assay was ...
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Implementation of a process internal control to monitor the performance of real-time PCR assays for the detection of
Vibrio cholerae in waterThe process control utilised in this study was an Escherichia coli (E. coli-GFP) strain carrying a chromosomally integrated copy of the green fluorescent protein (gfp) gene of the jelly fish Aequorea victoria. Application of the process control involved spiking samples with the E. coli-GFP containing cells and detecting gfp using real-time polymerase chain reaction (PCR). The process control ...
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Microbiotests for Cyanobacterial Toxins Screening
Abstract : The development of massive blooms of cyanobacteria (blue-green algae) in eutrophicated fresh and salt waters is an actual problem in many countries. Moreover, many strains of cyanobacteria produce toxins with a specific mode of action, the most studied being hepatotoxins (such as e.g. microcystin) and neurotoxins (e.g. anatoxin-a). Toxicity testing of crude extracts of cyanobacterial ...
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