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Chromatography Columns Applications

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    Chromatography laboratory instrumentation for environmental analysis

    Determining pollutants in environmental and food matrices requires efficient sample preparation and analytics. BUCHI provide a wide selection of solutions for reliable, flexible and safe sample processing procedures in the laboratory work flow for environmental analysis.

    By BÜCHI Labortechnik AG based in Postfach, SWITZERLAND.

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    Chromatography laboratory instrumentation for pharmaceutical

    Drug discovery and development for pharmaceutical industries require safe, efficient and compliant sample preparations and analytics. BUCHI offers a variety of reliable solutions for regulated pharmaceutical laboratories.

    By BÜCHI Labortechnik AG based in Postfach, SWITZERLAND.

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    Chromatography laboratory instrumentation for chemical

    Cost effective, safe and sustainable processes play a key role in the chemical industry. To cover those needs BUCHI offers a variety of reliable solutions for R&D, quality control and production.

    By BÜCHI Labortechnik AG based in Postfach, SWITZERLAND.

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    Determination of Additives in Beverages using Ascentis Express Columns

    Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications using Ascentis Express HPLC columns. Ascentis Express columns offer faster HPLC on any system. One benefit is their ability to produce the resolution, efficiency, and speed on conventional HPLC systems that is associated with the use of sub-2 micron columns on a UHPLC system, without generating high backpressure. Column chemistries (RP-Amide and HILIC) were selected for this article based on their enhanced performance with polar compounds in comparison to C18.

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Increased Pigment Removal with Analysis of Pesticides in Oranges

    An application was developed to demonstrate increased pigment removal for the analysis of pesticides, using Supel™ QuE Z-Sep/C18 QuEChERS and an Ascentis® Express C18 HPLC Column. The improved cleanup can decrease column and instrument fouling, leading to extended LC column lifetime and reduced instrument downtime.

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Chromatography laboratory instrumentation for feed (pet food)

    To produce efficiently several feed formulations starting from continuously changing raw materials and premixes, require flexible and accurate analytical and formulation technologies. BUCHI offers dedicated solutions for the entire value chain: from R&D and QC Laboratories to the production lines.

    By BÜCHI Labortechnik AG based in Postfach, SWITZERLAND.

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    Analysis of VOC compounds in water by Purge & Trap or dynamic headspace

    Context & Challenges Volatile organic compounds (VOC) can be dissolved in water. Sometimes water is polluted and to study contamination of VOC in finished drinking water, VOC must be measured. Chromatotec® Solutions Chromatotec® has developed a specific system for the measurement of VOC in water: a Purge & Trap system, the airmoPURGE. The analyzer is coupled with a degasing system allowing the extraction of dissolved gases. The complete system is based on: - 502.2 method from US EPA – “Volatile organic compounds in water by purge and trap capillary column gas chromatography with photoionization and electrolytic conductivity detectors in series – Revision 2.1”. - 524.2 method from US EPA – “Measurement of purgeable organic compounds in water by capillary column gas chromatography/mass spectrometry – Revision 4.0”.

    By Chromatotec Group based in Val de Virvée, FRANCE.

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    Analysis of Isocyanates Using the ASSET™ EZ4-NCO Dry Sampler

    Isocyanates are used as a raw material to produce a number of products . Personal exposure can occur while the products made with isocyanates are being applied, or when the materials are removed by grinding or thermal degradation. Workers who are exposed to these compounds are at risk for respiratory disorders and asthma. The highly reactive nature of the isocyanate compounds and the low occupational exposure limits put high demands on both sampling and analytical techniques for monitoring of isocyanates in air. We investigated the performance of the LC-MS and LC-MS-MS methods in the analysis of isocyanates using the new ASSET™ EZ4-NCO Dry Sampler. We found that the analytical method can successfully reach the quantitation limit for most isocyanates of 5 ng/mL in the final sample when LC-MS-MS analysis was used and the quantitation limit of 10 ng/mL when LC-MS analysis was used. All 11 compounds were well-resolved chromatographically using a 15 cm Ascentis Express C18 column.

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Reduced Fat Interference with Analysis of Pesticides in Avocados

    QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) is a very popular method for the extraction and cleanup of fruit and vegetable samples for pesticide residue analysis.2-4 The extraction step uses acetonitrile and a salting out effect (with MgSO4). Cleanup is then accomplished using dispersive SPE (dSPE) followed by a centrifuge step prior to analysis. This application shows the clean-up and analysis using Supel™ QuE Z-Sep+ QuEChERS and an SLB®-5ms GC column.

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Chromatography laboratory instrumentation for academia

    Education and research count on intuitive, flexible and expandable laboratory equipment. BUCHI possesses elaborated solutions for various steps in academic laboratories covering a wide range of applications.

    By BÜCHI Labortechnik AG based in Postfach, SWITZERLAND.

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    Chromatography laboratory instrumentation for food & beverage

    Effective measures against food fraud, efficient and healthy food processing and the development of functional food and neutraceuticals are just few of many challenges in modern F&B production and analysis. BUCHI offers classical reference methods and near infrared spectroscopy (NIRS) for food analysis, but also visualization of production processes using spray drying and encapsulation.

    By BÜCHI Labortechnik AG based in Postfach, SWITZERLAND.

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    Measurement of methane, methanol and ethanol for the biomass monitoring for biofuels.

    The Series 8900 provides direct measurement of methane, methanol, and ethanol for the biofuel industry. The Series 8900 GC employs a flame ionization detector (FID). The methane, methanol, and ethanol in the gas sample are physically separated using proprietary GC columns. A dual-column configuration with timed backflush to vent is used to strip off moisture and heavier gases. At sample injection, a fixed volume of sample is carried through the pre-cut column. The backflush is timed so that primarily the MME and other similar compounds continue on to the analytical column. Contaminants are then backflushed to vent. Methane, methanol, and ethanol are separated from potentially interfering components on the analytical column and elute to the detector for analysis.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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    Measurement of methyl ethyl ketone, chloroform & carbon tetrachloride in air for remediation & super fund sites

    The Series 8900 Analyzer provides direct measurement of Methyl Ethyl Ketone, Chloroform and Carbon Tetrachloride in ambient air. This instrument is utilized for remediation and Super Fund sites and to monitor workplace exposure limits.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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    Measurement of C1-C5 with carbon dioxide for oil & gas exploration.

    The PetroAlert Model 8900 combines the selectivity of gas chromatography with the sensitivity, broad dynamic range, and hydrocarbon selectivity of a flame ionization detector (FID) providing fast analysis of C1-C5 hydrocarbons and Carbon Dioxide required in Mud Logging for Oil & Gas exploration. The PetroAlert Model 8900 uses a dual column configuration with timed backflush to vent which is used to strip off moisture and C6+ hydrocarbons. A pre-cut column is used in series with the analytical column. At sample injection a fixed sample volume is carried to the pre-cut column. Backflush of the pre-cut column is timed from sample injection so that only C1-C5 hydrocarbons are eluted from the pre-cut column to the analytical column. C6+ hydrocarbons on the pre-cut column are then vented.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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    BTEX in Ambient Air Application using a High-Sensitivity PID

    The Series 8900 BTEX Analyzer with a high-sensitivity PID provides direct low level measurement of Benzene, Toluene, Ethylbenzene, and Xylene`s in ambient air. This instrument is utilized in ambient air networks around metropolitan areas and fence-line monitoring at industrial sites.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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    Flow meters for the general research flow measurement solutions

    Supporting research and production worldwide for over 40 years.  Mass flow meters and controllers give you the advantage with precision data and application flexibility.

    By Sierra Instruments, Inc. based in Monterey, CALIFORNIA (USA).

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    Measurement of acetaldehyde, methanol, & benzene in CO2 applications for gas manufacturers & food & beverage industry

    The BevAlert Model 8900 provides direct measurement of Acetaldehyde, Methanol, and Benzene (AMB) in Carbon Dioxide. The instrument is utilized by Specialty Gas Manufacturers and the Food and Beverage Industry to monitor trace impurities in CO2 used in carbonated beverages. The BevAlert Model 8900 employs a photoionization detector (PID). The AMB in the gas sample are physically separated using proprietary GC columns. A dual-column configuration with timed backflush to vent is used to strip off moisture and heavier gases. At sample injection, a fixed volume of sample is carried through the pre-cut column. The backflush is timed so that primarily the AMB and other similar compounds continue on to the analytical column. Contaminants are then backflushed to vent. Acetaldehyde, Methanol, and Benzene are separated from potentially interfering components on the analytical column and elute to the detector for analysis.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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    Measuring aromatic hydrocarbons (BTEX) for pollution control and environmental monitoring

    The Series 8900 BTEX Analyzer provides direct measurement of Benzene, Toluene, Ethylbenzene, and Xylenes in ambient air. This instrument is utilized in ambient air networks around metropolitan areas and fence-line monitoring at industrial sites. The Series 8900 BTEX Analyzer employs a photoionization detector (PID) as the sensing element. This detector is specific to volatile organic compounds. The Benzene, Toluene, Ethylbenzene, and Xylene`s in the gas sample are physically separated using proprietary GC columns.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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    Measurement of hydrogen sulfide & carbon disulfide in air for continous emission monitoring

    The Series 8900 Hydrogen Sulfide and Carbon Disulfide Analyzer provides direct measurement of H2S and CS2 in ambient air. This instrument is utilized for continuous emission monitoring. The Series 8900 H2S and CS2 Analyzer employs a Photoionization detector (PID) as the sensing element. A dual column configuration with timed backflush to vent is used to strip off moisture and heavier hydrocarbons. A pre-cut column is used in series with the analytical column. At sample injection a fixed volume of sample is carried to the pre-cut column. Backflush is timed so that only the H2S, CS2, and other similar components are eluted to the analytical column. Contaminants on the pre-cut column are backflushed to vent. H2S and CS2 are separated from potentially interfering components on the analytical column and elute to the detector for analysis.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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    Measurement of C1-C5 hydrocarbons for oil & gas exploration.

    The PetroAlert Model 8900 combines the selectivity of gas chromatography with the sensitivity, broad dynamic range, and hydrocarbon selectivity of a flame ionization detector (FID) providing fast analysis of C1-C5 hydrocarbons required in Mud Logging for Oil & Gas exploration. The PetroAlert Model 8900 uses a dual column configuration with timed backflush to vent which is used to strip off moisture and C6+ hydrocarbons. A pre-cut column is used in series with the analytical column. At sample injection a fixed sample volume is carried to the pre-cut column. Backflush of the pre-cut column is timed from sample injection so that only C1-C5 hydrocarbons are eluted from the pre-cut column to the analytical column. C6+ hydrocarbons on the pre-cut column are then vented. C1-C5 hydrocarbons are separated on the analytical column and elute to the flame ionization detector (FID) for analysis.

    By AMETEK MOCON - Baseline based in Lyons, COLORADO (USA).

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