Chromatography Columns Articles

  • Waters HPLC & UHPLC Application Notes at Your Finger Tips

    Summary A quick reference notebook to Waters HPLC/UHPLC application note highlights.   140 application notes covering a wide range of markets, such as Pharma, Food, and Health Science. Condensed one page format highlighting experimental conditions and chromatographic results. Tips on how to choose the best column to deliver ...


    By Waters Corporation

  • A prototype of novel agro-waste based column bed device for removal of textile dye Optilan Red

    The aim of the study was to assess the potentiality of an agro-waste (sugarcane bagasse) for removal of the textile dye (Optilan Red) using novel column based filtration unit with a packed column of chemically treated sugarcane bagasse. The treated and untreated sugarcane bagasse (biosorbent) were characterized by Fourier transform infrared (FT-IR) spectroscopy. Effect of initial dye ...


    By IWA Publishing

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • Update on Guard Cartridges for Trident Holders

    You may have seen my last blog post about guard holders and cartridges that we have available for our various LC product lines, “Which guard cartridges and holders go with which LC analytical columns?” Along those lines of discussion, the following note is available to also show what specific products are currently offered for this: ...


    By Restek Corporation

  • Biosorptive removal of malachite green from aqueous solution using chemically modified brown marine alga Sargassum swartzii

    Sargassum swartzii, marine macro brown alga, showed a high malachite green (MG) biosorption capacity in batch mode of operation. The analytical evidence from Fourier transform infrared spectra confirmed the involvement of amine group in the biosorption of MG and electrostatic interaction type of mechanism was proposed to occur between the amine group of dye and the cationic MG dye ...


    By IWA Publishing

  • A brief introduction to DiatoSorb-W

    DiatoSorb-W is a diatomaceous earth material that Restek will sell in bulk and in packed columns, uncoated and coated (with a liquid phase of your choice), which will replace Chromosorb-W packings. For those of you who may not know, the “W” stands for “white”, and is simply the color of the untreated, uncoated material. If your method references USP S1A, or Chromosorb-W/AW ...


    By Restek Corporation

  • Modeling of breakthrough curves for aqueous iron (III) adsorption on chitosan-sodium tripolyphosphate

    A fixed bed column packed with chitosan-sodium tripolyphosphate (CTPP) beads was used to remove aqueous Fe (III) ions. The adsorption of Fe (III) ions on CTPP beads was found to be dependent on operating conditions, such as the flow rate, adsorbent bed length, and feed concentration. The experimental data were assessed with Thomas, Adams-Bohart and Yoon-Nelson models to predict the ...


    By IWA Publishing

  • Separation of quercetin from a Ginkgo Biloba mother tincture using the PrepChrom C-700

    The aim of this application note was to isolate a flavonol, quercetin, from a Ginkgo Biloba mother tincture. The prepurification of the mother tincture was performed on a reversed phase flash column by the means of dry loading. The target compound was identified by its characteristic UV-VIS spectra and the retention time determined by running a standard. Subsequently, the purification of the ...


    By BÜCHI Labortechnik AG

  • Research on mass transfer columns: passe?

    Since university research on mass transfer columns has decreased significantly in recent years, it is discussed whether the current knowledge of mass transfer technology for trays, packing materials, and column internals is sufficient to design energy- and cost-efficient systems. Points of view are described that have not yet been clarified from an industrial position and should play an important ...


    By Raschig USA Inc.

  • Chromatography plays its part in turning Science Fiction into Science Fact

    Tomek Bagiński’s short film Ambition picks up on a fundamental drive of human kind, a thirst for knowledge and understanding, a thirst that can be sated through projects like Rosetta which are turning science fiction into science fact: Ambition the film Rosetta is an ESA mission with contributions ...


    By Anthias Consulting Ltd.

  • Separation and identification of organic compounds from thermally dissolved Shengli lignite in a methanol/benzene mixed solvent

    Thermal dissolution of Shengli lignite was conducted in a methanol/benzene mixed solvent at 240°C for 1 h. The soluble fraction was sequentially eluted with petroleum ether (PE) and carbon disulfide (CDS)/PE mixed solvents with increased CDS to PE ratio through a silica gel–packed column. A series of normal alkanes, polycyclic aromatics, methyl alkanoates, alkanoic acids, and fatty acid amides ...


    By Inderscience Publishers

  • GC Determination of Benzene in Reformulated Gasoline using a Supelco SLB-IL111 Ionic Liquid Column

    The amount of benzene in gasoline is a concern because it is a known human carcinogen, and exposure to it has been linked to detrimental health effects.  The challenge with the analysis lies in the complex composition of gasoline, which consists of hundreds of different compounds.  This application shows the separation of benzene and other gasoline compounds using a 60 m x 0.25 mm I.D., ...


    By MilliporeSigma

  • Analysis of the Pesticides Carbaryl, Diuron, and Propachlor

    Application Report 208: Analysis of the Pesticides Carbaryl, Diuron, and Propachlor Using Ascentis™ C18 This application demonstrates the suitability of the Ascentis C18 for efficient separation of the pesticides carbaryl, diuron, and propachlor. The analysis is carried out using a 15 cm x 4.6 mm I.D Ascentis C18 column under isocratic ...


    By MilliporeSigma

  • Analyze Trace Amounts of Solvents in Water Using Packed Column GC

    Glass or stainless steel columns containing 80/120 Carbopack B/3% SP-1500 packing can be used to analyze water-based samples for ppm to low percentage levels of many organic compounds. This bulletin describes minimum detection limits and maximum capacity of these columns, adsorption and memory effects, and other factors that affect component quantitation. Problems are defined that might arise ...


    By MilliporeSigma

  • Analysis of Bisphenol A on Ascentis Express RP-Amide

    Analysis of Bisphenol A and Related Compounds on Ascentis™ Express RP-Amide This application report shows the efficient separation of Bisphenol A, Dimethyl Bisphenol A, and Nonylphenol, via HPLC using an Ascentis Express RP-Amide column, under gradient conditions and with UV detection. ...


    By MilliporeSigma

  • Analysis of Phenolic and Benzyl Compounds Using Ascentis™ RP-Amide

    Application Report 226 Phenolic and Benzyl Compounds Using Ascentis™ RP-Amide This application demonstrates the suitability of Ascentis RP-Amide for the efficient separation of phenolic and benzyl compounds by HPLC, under isocratic conditions and with UV detection.  Compounds analyzed include: Uracil, Phloroglucinol, Pyrogallol, Benzamide, Resorcinol, ...


    By MilliporeSigma

  • Ascentis™ C18 for the Analysis of Phenolic and Benzyl Compounds

    Application Report 225   Phenolic and Benzyl Compounds Using Ascentis™ C18 This application demonstrates the suitability of Ascentis C18 for the efficient separation of phenolic and benzyl compounds by HPLC. Compounds analyzed include: Uracil, Phloroglucinol, Pyrogallol, Benzamide, Resorcinol, Catechol, Phenol, and Nitrobenzene.  ...


    By MilliporeSigma

  • Analysis of trace hydrocarbon impurities in 1,3-Butadiene using optimized RT-Alumina BOND/MAPD plot columns

    Abstract Identifying and quantifying trace impurities in 1,3-butadiene is critical in producing high quality synthetic rubber products. Standard analytical methods employ alumina PLOT columns which yield good resolution for low molecular weight hydrocarbons, but suffer from irreproducibility and poor sensitivity for polar hydrocarbons. In this study, Rt-Alumina BOND/MAPD PLOT columns were ...


    By Restek Corporation

  • Lower Detection Limits with Ground-Breaking Column Technology

    Rxi technology unifies outstanding inertness, low bleed, and high reproducibility into a single high performance column line. Take variation out of the equation and get the most consistent results for trace-level analysis with Rxi columns. phases available Rxi-1ms Rxi-1HT Rxi-5ms Rxi-5Sil MS Rxi-5HT Rxi-XLB ...


    By Restek Corporation

  • Analyses of Permanent Gases, Light Hydrocarbons, and Light Polar Compounds, Using Packed Column GC

    GC columns containing Carboxen-1000 packing separate mixtures of permanent gases (nitrogen, hydrogen, oxygen/argon, carbon monoxide, carbon dioxide) and C1-C3 light hydrocarbons (methane, acetylene, ethylene, ethane, propyne, propylene, and propane). A 15’ x 1/8” column will separate the permanent gases and C1 and C2 hydrocarbons in less than 25 minutes. A 2’ x 1/8” column ...


    By MilliporeSigma

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