Chromatography Columns Articles

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • Webinar Recap: “Developing, Testing, and Troubleshooting Chromatography Method Transfer”

    Hi, I’m Tom Wheat, Principal Scientist at Waters, and I recently hosted a webinar entitled, “Developing, Testing and Troubleshooting Chromatography Method Transfer.” Over the course of the webinar, we discussed the transfer of chromatographic methods that occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one ...


    By Waters Corporation

  • High-Resolution Ultrahigh-Pressure Long Column Reversed-Phase Liquid Chromatography for Top-down Proteomics

    Separation of proteoforms for global intact protein analysis (i.e. top-down proteomics) has lagged well behind what is achievable for peptides in traditional bottom-up proteomic approach and is becoming a true bottle neck for top-down proteomics. Herein, we report use of long (≥1 M) columns containing short alkyl (C1-C4) bonded phases to achieve high-resolution RPLC for separation of ...

  • Some notes about Fats and Oils, cis/trans-Fatty acids and our latest re-launched RT 2560

    I recently posted a Blog regarding some notes about Fats and Oils, FAMES and our Famewax column, which can be found here. In Food Industry the challenge may be a bit heavier, if the amount of Trans Fatty Acids is requested. There is scientific consensus that trans fats intake has a negative effect on human health: more specifically, consumption of trans fats ...


    By Restek Corporation

  • Top 16 for 2016 - Restek LC article makes the cut

    If you are an avid chromatographer you likely subscribe to LC/GC magazine or its popular electronic version, E-Separation Solutions. It is definitely the place to go for news and information related to all things Chromatography. Over the years Restek has had many an article published in LC/GC and, full disclosure, we advertise there as well. Who in the business doesn’t? It is the place ...


    By Restek Corporation

  • Fluorophenyl LC Phases- what you should know

     They may be called PFP, PFPP, pentafluorophenyl, fluorophenyl or F5 phases. What they all have in common is the structure shown above (the pentafluorophenyl  group) attached to the silica particles. Most modern phases contain a propyl spacer between the above group and the silica, so that the phase looks something like this: ...


    By Restek Corporation

  • Photocatalytic degradation of tartrazine dye using CuO straw-sheaf-like nanostructures

    Straw-sheaf-like CuO nanostructures were fruitfully synthesized using a chemical precipitation approach for the photocatalytic degradation assessment of tartrazine. Phase identification, composition, and morphological outlook of prepared CuO nanostructures were established by X-ray diffraction and scanning electron microscopy analysis. The photocatalytic performance of the synthesized CuO ...


    By IWA Publishing

  • Update on Guard Cartridges for Trident Holders

    You may have seen my last blog post about guard holders and cartridges that we have available for our various LC product lines, “Which guard cartridges and holders go with which LC analytical columns?” Along those lines of discussion, the following note is available to also show what specific products are currently offered for this: ...


    By Restek Corporation

  • Separation of citral isomers from lemongrass oil

    Reveleris® Prep: Purification with flash chromatography followed by a prep-HPLC isolation of citral isomers Lemongrass is cultivated around the world. It's mainly grown for its oils, which have a number of uses including for vitamin A, perfumes, insect spray, cosmetics, perfumes, food and drinks. Lemongrass is also enjoyed as a tea throughout the world. Lemongrass oil contains several ...


    By BÜCHI Labortechnik AG

  • Why Biphenyl should be your first choice in LC.. A perspective from a “ GC” specialist..

    Being in GC for 38 years it made me wonder why in LC mostly C18 columns are used for developing a method. I never understood this as its against the basic rules for selecting the optimal stationary phase. In LC we see that about 95% of the applications is done using reversed phase materials, especially the C18 type. There are many C18 types available, and they all show a different separation ...


    By Restek Corporation

  • Biosorptive removal of malachite green from aqueous solution using chemically modified brown marine alga Sargassum swartzii

    Sargassum swartzii, marine macro brown alga, showed a high malachite green (MG) biosorption capacity in batch mode of operation. The analytical evidence from Fourier transform infrared spectra confirmed the involvement of amine group in the biosorption of MG and electrostatic interaction type of mechanism was proposed to occur between the amine group of dye and the cationic MG dye ...


    By IWA Publishing

  • Development of a Cleaning Validation Method for MICRO® A07 Citric Acid Cleaner Using HPLC-CAD

    Abstract A high performance liquid chromatography (HPLC) method with a Corona Charged Aerosol detector for the cleaning validation of MICRO* A07 Citric Acid Cleaner residue has been developed. The pooled average repeatability of 3 replicates at 6 concentration levels expressed as RSD was 1.3 % while the pooled average intermediate precision (intra-day repeatability in 3 days) of 3 replicates ...

  • A brief introduction to DiatoSorb-W

    DiatoSorb-W is a diatomaceous earth material that Restek will sell in bulk and in packed columns, uncoated and coated (with a liquid phase of your choice), which will replace Chromosorb-W packings. For those of you who may not know, the “W” stands for “white”, and is simply the color of the untreated, uncoated material. If your method references USP S1A, or Chromosorb-W/AW ...


    By Restek Corporation

  • Case study - Veolia MPPE technology, Inpex Ichthys LNG - Australia

    Inpex Ichthys Project, Browse Basin, 220 km of Western Australia 8.9 million tons LNG/year; 1.6 million tons LPG/year; 100.000 barrels Condensate/day. Central Processing Facility on Semi-Submersible Platform separates Condensate and Gas. Gas is sent by pipeline to Darwin. Condensate is stored on FPSO. The MPPE unit is located on the FPSO. ...

  • Comparison of simple ozonation and direct hydrogen peroxide processes in TNT removal from aqueous solution

    Oxidation of 2,4,6-trinitrotoluene (TNT) using simple ozonation and direct hydrogen peroxide was studied. An analytical method based on the HPLC (high performance liquid chromatography) system was used for TNT detection. The influence of parameters such as pH of the solution (3–10), initial TNT concentration (10–100 mg/L), dosage of ozone (0.2–1 g/hr), H2O2/TNT molar ratio (250–1,000), ...


    By IWA Publishing

  • Removal of perfluorooctanoic acid (PFOA) in groundwater by nanofiltration membrane

    Perfluorooctanoic acid (PFOA) is very persistent in the environment and resistant to typical degradation processes. PFOA has been widely used in surface-active agents and as an emulsifier in several products and can contaminate groundwater. Groundwater is considered as an important source of water; hence removal of PFOA contamination in groundwater is needed. This study aimed to examine the ...


    By IWA Publishing

  • Modeling of breakthrough curves for aqueous iron (III) adsorption on chitosan-sodium tripolyphosphate

    A fixed bed column packed with chitosan-sodium tripolyphosphate (CTPP) beads was used to remove aqueous Fe (III) ions. The adsorption of Fe (III) ions on CTPP beads was found to be dependent on operating conditions, such as the flow rate, adsorbent bed length, and feed concentration. The experimental data were assessed with Thomas, Adams-Bohart and Yoon-Nelson models to predict the ...


    By IWA Publishing

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • Is Microflow LC a Reality or Perception?

    Microflow or nanoflow chromatography has been used mostly in the academic and fundamental research environment in the past for its various advantages over analytical liquid chromatography, but rarely in the industrial settings. This is most likely because many of us believe it is a pain-in-the-neck technique to perform! But is this a reality or just a ...


    By Waters Corporation

  • Method Scaling in Preparative HPLC: Analytical Method & Preparative Method

    VOC:  “I tried to scale up my analytical HPLC method to prep and it didn’t work.” Andy:  This is one of the most common things we hear from chemists wanting to do preparative chromatography. We also hear the following question pretty often too: VOC:  “I have a good analytical HPLC method, how do I scale it to ...


    By Waters Corporation

Need help finding the right suppliers? Try XPRT Sourcing. Let the XPRTs do the work for you