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Chromatography Applications

  • Percent Aromatics in Gasoline #3008

    The aromatic content of gasoline determines many of its combustion properties. Since it also impacts the environmental characteristics of the fuel it is desirable to have accurate measurements of this parameter. The traditional analytical method for measuring aromatics is either gas chromatography (GC) or an older method entitled fluorescent indicator adsorption (FIA), both of which are time and labor intensive. This note will discuss the use of Guided Wave hardware and software tools for the measurement of % aromatics in fuel products using fiber optic-based, Near-Infrared (NIR) spectroscopy. NIR can be applied in real time directly in process monitoring or as a laboratory procedure. In either case NIR is a time and money saving alternative to traditional methods. Request Application Note #3008

    By Guided Wave Inc, based in Rancho Cordova, CALIFORNIA (USA).

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    Analysis of Herbicides in Drinking Water

    Herbicides are used throughout the world to combat the growth of unwanted plant life. These polar compounds are hydrophilic, so they may find their way into drinking water sources. While gas chromatography has historically been the preferred technique for analysis of herbicides in water samples, the use of LC-MS/MS for this application is gaining acceptance. This is due to the power of MS/MS to provide detailed identification of multiple analytes, but also in part due to the variety of HPLC stationary phases that are available. In particular, several HPLC phases are available that provide great retention and peak shapes for polar analytes, such as herbicides, even under mostly aqueous mobile phase conditions. The combination of these factors may possibly eliminate need for time-consuming sample preparation.

    By Sigma-Aldrich Co., LLC based in Bellefonte, PENNSYLVANIA (USA).

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    Increased Pigment Removal with Analysis of Pesticides in Oranges

    An application was developed to demonstrate increased pigment removal for the analysis of pesticides, using Supel™ QuE Z-Sep/C18 QuEChERS and an Ascentis® Express C18 HPLC Column. The improved cleanup can decrease column and instrument fouling, leading to extended LC column lifetime and reduced instrument downtime.

    By Sigma-Aldrich Co., LLC based in Bellefonte, PENNSYLVANIA (USA).

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    Mass flow devices for analytical instrumentation

    Axetris mass flow devices are used in a variety of analytical applications: Gas Chromatography, Mass Spectrometry, Atomic Spectroscopy, Elemental Analysis, Thermal Analysis, Bioreactor and Fermentor.

    By Axetris Ag based in Kaegiswil, SWITZERLAND.

  • Chromatography systems for environmental monitoring

    With the growth of contamination generated from agricultural and industrial production, it causes a threat to the enviroment including drinking water, atmosphere and soil. The chemists have always seeked out the effective analytical methods to detect and identify chemical contaminants. Dikma provides innovative products and application solutions according to government regulations and industry standards, for air and water quality monitoring, gas detection.

    By Dikma Technologies Inc. based in Lake Forest, CALIFORNIA (USA).

  • Gas chromatography for laboratories

    By F-DGSi based in Evry Cedex, FRANCE.

  • Liquid Chromatography for soil consultancy

    Everyone has a responsibility to care for the environment to reduce the levels of pollution, including businesses that daily release emissions into the air and the ground. This is why we provide a full-service environmental testing service to help businesses identify problems and resolve them so they operate in the most environmentally-friendly method possible. We offer air emission testing, emissions monitoring services and stack emissions testing to businesses of all sizes.

    By Custom Stack Analysis, LLC. based in Alliance, OHIO (USA).

  • Alkane (hydrocarbon) gas measurement for mudlogging in oil & gas drilling rigs

    The need for operator and maintainance intensive gas chromatograph (GC) analyzers was eliminated. Customer can now deploy all-optical, unattended sensor system for monitoring the composition of hydrocarbon gases (C1-C5) exiting the well bore which provides crucial information about the drilling formation that would save time and money.

    By MKS Instruments, Inc. based in Andover, MASSACHUSETTS (USA).

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    Determination of Additives in Beverages using Ascentis Express Columns

    Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications using Ascentis Express HPLC columns. Ascentis Express columns offer faster HPLC on any system. One benefit is their ability to produce the resolution, efficiency, and speed on conventional HPLC systems that is associated with the use of sub-2 micron columns on a UHPLC system, without generating high backpressure. Column chemistries (RP-Amide and HILIC) were selected for this article based on their enhanced performance with polar compounds in comparison to C18.

    By Sigma-Aldrich Co., LLC based in Bellefonte, PENNSYLVANIA (USA).

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    Reproducible Recoveries of Pesticides from Fruits and Vegetables

    A group of 30 compounds was selected, representing pesticides, acaricides, insecticides, and fungicides. These samples were analyzed using Supel™ QuE QuEChERS and an Ascentis® Express RP-Amide HPLC Column.

    By Sigma-Aldrich Co., LLC based in Bellefonte, PENNSYLVANIA (USA).

  • Filtration systems & solutions for biosciences and scientific industry

    The scientific community and in particular for that of the life science industries such as the medical, pharmaceutical  and healthcare, have a growing need for superior technologies and products in filtration, separation, purification, aeration and liquid transfer. The range of BioVyon materials and applications are continuously expanding, driven by new market requirements and available to both OEM and end user customers. Applications include separation or filtration for healthcare, medical and pharmaceutical products, shut off valves for catheters, diagnostic wick and sample preparation devices.

    By Porvair Filtration Group based in Fareham, UNITED KINGDOM.

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    Improved Reproducibility and Reduced Sample Preparation Time for the HPLC Analysis of Aflatoxins in Raw Peanut Paste

    The US FDA and international regulatory agencies have set contamination levels for aflatoxins in animal feedstuffs. Since Aspergillus may infect commodities pre-harvest, during storage or during processing, monitoring for aflatoxins in associated agricultural commodities at all stages of production is requisite. Field screening methods exist that are adequate to estimate contamination levels for aflatoxins. When additional confirmation or quantification is desired, chromatographic laboratory analysis is often necessary. Preparation of matrix samples prior to chromatographic analysis typically requires extraction and purification. Commonly, immunoaffinity columns (IAC), which employ a multi-step bind and elute mechanism to concentrate and purify aflatoxins, are used to purify matrix samples for subsequent analysis. Solid phase extraction (SPE), an alternate method which may use interference removal, can also be employed.

    By Sigma-Aldrich Co., LLC based in Bellefonte, PENNSYLVANIA (USA).

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    Formaldehyde and Acetaldehyde Determination in Air Using Fully Automated On-Line Desorption and Analysis of DNPH Cartridges

    Airborne aldehydes and ketones are collected by passing air through a cartridge containing 2,4-dinitrophenylhydrazine (DNPH). Carbonyl compounds react with the DNPH to form hydrazones, which are immobilized on the cartridge. These compounds can be easily eluted from the cartridge with acetonitrile and analyzed by HPLC with UV detection. Traditionally, this analysis including the workup contains a series of manual steps, which can become time-consuming and could incur experimental error. Automating the extraction of LpDNPH S10 cartridges and putting it in-line with the HPLC analysis will significantly reduce manual labor using this technique and this will improve reproducibility of the method by reducing potential experimental errors by the operator. The automation and unattended operation of the method leads to high throughput for determining airborne formaldehyde and acetaldehyde.

    By Sigma-Aldrich Co., LLC based in Bellefonte, PENNSYLVANIA (USA).

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    Measurement of oil content of oil bearing algae used for biofuel production

    We assist the rapidly growing global biofuels industry through our MQC analysers, speeding up analyses by up to 250 times, and dispensing with the need for hazardous solvents and skilled operators required for traditional methods such as soxhlet extraction and chromatography. Benchtop, non-destructive single seed analysers enable seed breeders to select the highest oil bearing single seeds, accelerating the breeding of high oil bearing seeds for biofuel purposes by up to five years. Typical seeds are Jatropha and Camelina; our breadth of applications capability allows almost all seeds to be characterised. Benchtop seed analysers allow seed co-operatives and biofuel crushers/processors rapidly and accurately to measure the oil and moisture content of biofuel seeds. This enables farmers to be paid according to oil content, and crushing/oil processing to be optimised for the oil and moisture content to achieve the highest quality biofuel output.

    By Oxford Instruments plc based in Abingdon, UNITED KINGDOM.

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    Fast C1 to C5 - PetroAlert Series 9100

    The PetroAlert® Series 9100 combines the selectivity of gas chromatography with the sensitivity, broad dynamic range, and hydrocarbon selectivity of a flame ionization detector (FID) providing a 30 second analysis of C1-C5 hydrocarbons required in Mud Logging for Oil & Gas exploration, while maintaining C1-C2 separation at 200:1 concentration ratios.

    By MOCON, Inc. - Baseline based in Lyons, COLORADO (USA).

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    Gas analysis & monitoring system for gas purity

    The purity of a gas is an indication of the amount of other gases contained in it. A high purity refers to a low amount of other gases. High purity gas are considered to be of better quality and are generally more expensive. The purity of the gas can be expressed as a percentage or as a decimal fraction. Applications for checking the purity of the gases across different industrial sectors by air separation plants, cryogenic facilities, the quality control of industrial gases to the steel mills, etc.

    By ETG Risorse e Tecnologia based in Montiglio, ITALY.

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