DNA detection Articles
-
Biosensors - Using DNA as a biosensor to detect contaminants in water
Biosensor - Using DNA to detect water contamination. ...
-
Silicon optocouplers for biosensing
Here we review on the fabrication and the bioanalytical applications of a monolithic silicon optocoupler that integrates an array of silicon avalanche diodes as light sources, detectors and silicon nitride optical fibres, all intercoupled through a self-alignment technique. The optocoupler is combined with a microfluidic module that allows for simultaneous real-time sensing of multiple analytes. ...
-
Distinct functions of pot1 at telomeres
The mammalian protein POT1 binds to telomeric single-stranded DNA (ssDNA), protecting chromosome ends from being detected as sites of DNA damage. POT1 is composed of an N-terminal ssDNA-binding domain and a C-terminal protein interaction domain. With regard to the latter, POT1 heterodimerizes with the protein TPP1 to foster binding to telomeric ssDNA in vitro and binds the telomeric ...
-
Impact parameters investigation of DNA immobilisation process on DNA sensor response
This paper studies impact parameters of DNA sequence immobilisation on DNA sensor response. The parameters were examined including immobilisation time, carbon nanotubes concentration, DNA probe concentration and pH value. The DNA probe sequences were immobilised on surface of microelectrodes by using multi–walled carbon nanotubes (MWCNTs) as mediator. The hybridisation between DNA probe and DNA ...
-
Effect of low doses and low dose-rates of gamma radiation on DNA damage in human peripheral blood leukocytes using comet assay
Comet assay is a sensitive technique and it provides a unique opportunity to investigate intercellular differences in DNA damage and repair in any eukaryotic cell population. This assay can be used to detect DNA single strand breaks, alkali-labile sites and incomplete DNA repair sites, under alkaline conditions and DNA double strand breaks under neutral condition. We report here our observations ...
-
Potential applications of next generation DNA sequencing of 16S rRNA gene amplicons in microbial water quality monitoring
The applicability of next generation DNA sequencing (NGS) methods for water quality assessment has so far not been broadly investigated. This study set out to evaluate the potential of an NGS-based approach in a complex catchment with importance for drinking water abstraction. In this multi-compartment investigation, total bacterial communities in water, faeces, soil, and sediment samples ...
-
Specific PCR Assays for the Detection and Quantification of DNA from the Biocontrol Strain Trichoderma harzianum 2413 in Soil
Strain identification in situ is an important factor in the monitoring of microorganisms used in the field. In this study, we demonstrated the use of sequence-characterized amplified region (SCAR) markers to detect genomic DNA from Trichoderma harzianum 2413 from soil. Two primers (SCAR A1/SCAR A1c) were tested against DNA of 27 isolates of Trichoderma spp. and amplified a 990-bp fragment from T. ...
-
Surfaced Enhanced Raman Scattering (SERS)
Introduction Surfaced enhanced Raman spectroscopy, (SERS), is an enhancement technique used to overcome the challenges seen with weak Raman scattering. SERS provides all the advantages of Raman spectroscopy whilst also offering higher sensitivity thorough scattering enhancement and fluorescence quenching. SERS can increase the Raman signal by up to 1010-15, allowing the analysis of single ...
-
ANDalyze DNA sensor Technology
Video describing the use of DNA as a sensor for detecting heavy metal ions in ...
-
Plastic Makes the Difference: Ideal Real-time PCR Signals with Optimized Plastic Products
Plastic Makes the Difference: Ideal Real-time PCR Signals with Optimized Plastic Products Various microplates are available for quantitative real-time PCR in 96 well format. The main difference is the color, which significantly influences the real-time PCR signal quality and thus the results. A wide range of microplates from various manufacturers is available for real-time PCR in 96 well ...
-
Genotoxicity & Environmental Pollution
The Comet assay is playing an increasing role in studies to determine possible links between environmental pollutants and DNA integrity. The assay is an ideal biomarker for such investigations due to its sensitivity and reliability in detecting DNA damage and repair. Many research groups use our Comet Assay scoring systems as part of their efforts to determine the ...
By Instem Group
-
Applications of Peptide Nucleic Acid (PNA)
Peptide Nucleic Acid (PNA) is a synthetic polymer similar to DNA or RNA. PNA can be said to have been born in Denmark, where it was originally developed by Peter E. Nielsen (University of Copenhagen), Michael Egholm (University of Copenhagen), Rolf H. Berg (Risø National Laboratory), Technical University of Denmark) and Ole Buchardt (University of Copenhagen) invented it in 1991.Applications of ...
By BOC Sciences
-
Evaluating the insecticidal genes and their expressed products in bacillus thuringiensis strains by combining pcr with mass spectrometry
By a combination of PCR and mass spectrometry, a total of five cry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab, and cry1Ia) were detected in genomic DNA from the wild-type Bacillus thuringiensis strain 4.0718, and three protoxins (Cry1Aa, Cry1Ac, and Cry2Aa) were identified in the strain's parasporal crystals. These results indicated that this complementary method may be useful in evaluating B. ...
-
Rapid detection of pathogenic bacteria in surface water by PCR with universal primer
A rapid PCR method was developed by utilising a pair of universal primers which targeted four bacteria strains, namely Shigella dysenteriae, Vibrio cholerae, Salmonella typhimurium and Escherichia coli. The bacteria from several water bodies in the urban area of Xi'an City were detected by PCR, and the comparison was made with the conventional method. As a result, the detection limit of the DNA ...
-
Discrimination of gastric cancer from normal by serum RNA based on surface-enhanced Raman spectroscopy (SERS) and multivariate analysis
I. INTRODUCTION Currently gastric cancer is the second leading cause of cancerassociated death; accounting for approximately 600 000 annual deaths worldwide.1 The patients with gastric cancer have poor survival rates mainly due to the advanced stages upon the initial time of diagnosis when cancer cells have metastasized into other parts of the body. Early diagnosis and localization of malignant ...
-
Assessment of diversity of adenovirus DNA polymerase gene in recreational waters facilitated by ultracentrifugal concentration
Adenoviruses (AdV) are related to respiratory and gastrointestinal diseases in animals and human beings. Their wide genetic diversity in water bodies and their resistance to environmental conditions allow the use of AdV as a reliable marker for detection of fecal contamination. In this work, the diversity of AdV along Belo Stream – in the city of Caxias do Sul, Rio Grande do Sul, Brazil – was ...
-
A pcr-based method for monitoring legionella pneumophila in water samples detects viable but noncultivable legionellae that can recover their cultivability
Legionella pneumophila is the causative agent of Legionnaires' disease. This bacterium is ubiquitous in aqueous environments and uses amoebae as an intracellular replicative niche. Real-time PCR has been developed for rapid detection of Legionella DNA in water samples. In addition to culturable bacteria, this method may also detect dead and viable but noncultivable (VBNC) legionellae. In order to ...
-
Evaluation of alternative DNA extraction processes and real-time PCR for detecting Cryptosporidium parvum in drinking water
USEPA Method 1623 is the standard method in the United States for the detection of Cryptosporidium in water samples, but quantitative real-time polymerase chain reaction (qPCR) is an alternative technique that has been successfully used to detect Cryptosporidium in aqueous matrices. This study examined various modifications to a commercial nucleic acid extraction procedure in order to enhance ...
-
Assessment of molecular methods as a tool for detecting pathogenic protozoa isolated from water bodies
Several species belong to the Cryptosporidium and Giardia genus, the main parasitic protozoa occurring in water, but only some of them are infectious to humans. We investigated the occurrence of Cryptosporidium and Giardia and identified their species in the water samples collected from natural water bodies in north-western Poland. A total of 600 samples from water bodies used for bathing, ...
-
A novel DNA-based nanostructure for single molecule detection purposes
Recently, interest in DNA?nanoparticle hybrid structures synthesis for developing nanoscale assemblies with innovative diagnostics, optical and magnetic properties, all combined in one single nanostructure, is increasing. In other words, nanostructures, which show fluorescent and magnetic features simultaneously, are of particular interest for diagnostics objectives, especially genetic research ...
Need help finding the right suppliers? Try XPRT Sourcing. Let the XPRTs do the work for you