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Electrophoresis Applications

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    Determination of proteins concentrations and their molecular weights

    INTRODUCTION The method enables fast separation of proteins according to their molecular weights (Mw) with subsequent protein quantification in pr otein-containing samples.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of protein purity and heterogeneity with capillary gel electrophoresis and capillary isoelectric focusing

    INTRODUCTION Antibodies, protein-based therapeutics, and other recombinant proteins are final products in biotechnological and pharmaceutical industry. Determination of their purity, stability, and hetero geneity is of utmost importance since post-translational modifications as well as degradation processes can change drastically the biological activity of these proteins.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of serum protein fractions

    INTRODUCTION The method allows determination of relative amou nts of albumin and globulins in human serum using capillary electrophoresis methods.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of 4-carboxybenzaldehyde & p-toluic acid in purified terephthalic acid according to ASTM D7881 & ASTM D7882 test methods

    Introduction These test methods cover the determination of 4-carboxybenzaldehyde (4-CBA, 4-formylbenzoic acid) and p-toluic acid (p-TOL, 4-methylbenzoic acid) in purified terephthalic acid (PTA) by capillary electrophoresis (CE) with normal or reverse voltage mode and UV detection.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of hesperidin and naringin in juices using CAPEL-105M capillary electrophoresis system

    The method is intended for determination of hesperidin and naringin using capillary electrophoresis technique in order to reveal adulteration of juices. These components are tested in citrus juices, concentrated juices, nectars, juice-containing beverages, and fruit sauces that are produced primarily from oranges, grapefruits, tangerines, lemons, and limes.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of amino acids in beer and wort by capillary electrophoresis

    Free forms of amino acids in beer originate mostly from malt. The following method enables fast quantitative determination in beer and wort of the following free amino acids: arginine, lysine, tyrosine, phenylalanine, histidine, leucine and iso-leucine (total), methionine, valine, proline, alanine, glycine, cystine, tryptophan, aspartic and glutamic acids. Total content of amino acids is fdetermined after hydrolysis of proteins.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of dyes in beverages

    The method allows identification and determination of synthetic dyes in samples of soft and alcoholic drinks by capillary electrophoresis. The Capillary Electrophoresis (CE) method for the determination of synthetic dyes is based on their differential migration in a narrow quartz capillary under the influence of the applied electric field. Identification and quantitative determination of dyes is performed by measuring the UV absorbance at 254 nm (for “CAPEL® -103РТ/104Т” systems)...

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of phenoxycarboxylic acid herbicides in water using high performance capillary electrophoresys method using Capel systems

    The method allows determination of herbicides classified as phenoxycarboxylic acids, specifically: 2,4-dichloro-- phenoxybutyric acid (2,4-DB), 2,4-dichlorophenoxypropionic acid (2,4-DP, Dichlorprop), 2,4-dichloropheno-- xyacetic acid (2,4-D), and phenoxyacetic acid in samples of natural, potable and treated waste water by capillary electrophoresis.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of organic acids in beverages

    The method allows determination of organic acids, in soft drinks (juices, fizz drinks) and alcoholic drinks (wines, beer). The capillary electrophoresis method for evaluation of cations concentration is based on differential migration and separation of cations in electric field due to the difference in their electrophoretic mobility. Identification and quantitative determination of the analyzed cations is performed by indirect detection measuring UV absorption at 254 nm (for “CAPEL® -103RT/104T”...

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of Anions in Beverage

    Introduction The method is used for the determination of the mass concentration of chloride, sulfate, and nitrate in beverages by capillary electrophoresis. The method can be applied for all types of non-alcoholic and alcoholic beverages including sport and energetic drinks, juices, beer and beer products, wines, brandy and spirits, liquors, and vodka.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of D- and L-isomers of tartaric and malic acids in wines, wine materials, and food additive

    Naturally occurred is predominantly L-form of tartaric acid whereas its D-form and the relevant racemic mixture can be artificially synthesized. Generally, artificial acidification of wine with tartaric acid is allowed by OIV, but only pure L-form must be used for this purpose. Thus the analysis of tartaric acid isomers either in food additives or directly in wine can be an important parameter in clarifying any deviations in wine production. Analysis of isomers of malic acid is also within the necessary oenological...

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Identification and determination of synthetic dyes in soft and alcoholic drinks

    The method allows identification and determination of synthetic dyes in samples of soft and alcoholic drinks by capillary electrophoresis.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of inorganic cations in drinks

    The method enables fast determination of inorganic cations (sodium, potassium, calcium, and magnesium) in the samples of juices, beer, wines, brandies, brandy alcohols, and raw materials by capillary electrophoresis.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determnation of albumin concentration in urine

    INTRODUCTION The capillary electrophoresis method provides det ermination of albumin concentration in urine.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of cations in beverages

    The method enables fast determination of inorganic cations (sodium, potassium, calcium, and magnesium) in the samples of juices, beer, wines, brandies, brandy alcohols, and raw materials by capillary electrophoresis. The method for evaluation of cations concentration is based on differential migration and separation of cations in electric field due to the difference in their electrophoretic mobility. Identification and quantitative determination of the analyzed cations is performed by indirect detection measuring...

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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