Andreas Breidbach  and Wolfgang Brodacz [2,3,4] developed a new GC-MS method using fully 13C isotopelabelled analogues of T-2 toxin and HT-2 toxin that allows for the detection of these toxins at concentrations as low as 2–5 ppb.
An application was developed to demonstrate increased pigment removal for the analysis of pesticides, using Supel™ QuE Z-Sep/C18 QuEChERS and an Ascentis® Express C18 HPLC Column. The improved cleanup can decrease column and instrument fouling, leading to extended LC column lifetime and reduced instrument downtime.
The suitability of the new cleanup sorbent, Supel QuE Z-Sep+, has been successfully demonstrated for cleanup of beef kidney matrix. It was found to effectively reduce background without significantly reducing recovery of lipophilic pesticides such as organochlorines and some pyrethroids. GC-MS full-scan data indicated Z-Sep+ was found to provide better cleanup in the form of reduced background than PSA/C18, and less interference in the GC-MS/SIM analysis of the target pesticides themselves.
Caramel colorings are used as additives in a broad range of food and beverage products to impart a desired color, but have no nutritional or preservative function. Recently, the potential hazard to humans of
ammonia- and ammonia-sulfite-process caramel colorings was raised, because they contain the by-product 4-methylimidazole, which is a potential carcinogen.1 The methylimidazole compounds are difficult to analyze due to their polar nature and low molecular weight. Traditional reversed phase techniques are...
QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) is a very popular method for the extraction and cleanup of fruit and vegetable samples for pesticide residue analysis.2-4 The extraction step uses acetonitrile and a salting out effect (with MgSO4). Cleanup is then accomplished using dispersive SPE (dSPE) followed by a centrifuge step prior to analysis.
This application shows the clean-up and analysis using Supel™ QuE Z-Sep+ QuEChERS and an SLB®-5ms GC column.