HPLC Columns Applications

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  • Category: Monitoring & Testing ×

  • Subcategory: HPLC Columns ×

Increased Pigment Removal with Analysis of Pesticides in Oranges

by Sigma-Aldrich Co., LLC     based in Bellefonte, PENNSYLVANIA (USA)

An application was developed to demonstrate increased pigment removal for the analysis of pesticides, using Supel™ QuE Z-Sep/C18 QuEChERS and an Ascentis® Express C18 HPLC Column. The improved cleanup can decrease column and instrument fouling, leading to extended LC column lifetime and reduced instrument downtime.

Determination of Additives in Beverages using Ascentis Express Columns

by Sigma-Aldrich Co., LLC     based in Bellefonte, PENNSYLVANIA (USA)

Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications using Ascentis Express HPLC columns. Ascentis Express columns offer faster HPLC on any system. One benefit is their ability to produce the resolution, efficiency, and speed on conventional HPLC systems that is associated with the use of sub-2 micron columns on a UHPLC system, without generating high backpressure. Column chemistries (RP-Amide and HILIC) were selected for this article based on their enhanced performance with polar compounds in comparison to C18.

Reproducible Recoveries of Pesticides from Fruits and Vegetables

by Sigma-Aldrich Co., LLC     based in Bellefonte, PENNSYLVANIA (USA)

A group of 30 compounds was selected, representing pesticides, acaricides, insecticides, and fungicides. These samples were analyzed using Supel™ QuE QuEChERS and an Ascentis® Express RP-Amide HPLC Column.

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