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HPLC Applications

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    Increased Pigment Removal with Analysis of Pesticides in Oranges

    An application was developed to demonstrate increased pigment removal for the analysis of pesticides, using Supel™ QuE Z-Sep/C18 QuEChERS and an Ascentis® Express C18 HPLC Column. The improved cleanup can decrease column and instrument fouling, leading to extended LC column lifetime and reduced instrument downtime.

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Formaldehyde and Acetaldehyde Determination in Air Using Fully Automated On-Line Desorption and Analysis of DNPH Cartridges

    Airborne aldehydes and ketones are collected by passing air through a cartridge containing 2,4-dinitrophenylhydrazine (DNPH). Carbonyl compounds react with the DNPH to form hydrazones, which are immobilized on the cartridge. These compounds can be easily eluted from the cartridge with acetonitrile and analyzed by HPLC with UV detection. Traditionally, this analysis including the workup contains a series of manual steps, which can become time-consuming and could incur experimental error. Automating the extraction...

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Analysis of Herbicides in Drinking Water

    Herbicides are used throughout the world to combat the growth of unwanted plant life. These polar compounds are hydrophilic, so they may find their way into drinking water sources. While gas chromatography has historically been the preferred technique for analysis of herbicides in water samples, the use of LC-MS/MS for this application is gaining acceptance. This is due to the power of MS/MS to provide detailed identification of multiple analytes, but also in part due to the variety of HPLC stationary phases...

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Determination of Additives in Beverages using Ascentis Express Columns

    Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications...

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Analysis of Isocyanates Using the ASSET™ EZ4-NCO Dry Sampler

    Isocyanates are used as a raw material to produce a number of products . Personal exposure can occur while the products made with isocyanates are being applied, or when the materials are removed by grinding or thermal degradation. Workers who are exposed to these compounds are at risk for respiratory disorders and asthma. The highly reactive nature of the isocyanate compounds and the low occupational exposure limits put high demands on both sampling and analytical techniques for monitoring of isocyanates in...

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Analysis of Methylimidazoles in Caramel Colored Carbonated Beverages

    Caramel colorings are used as additives in a broad range of food and beverage products to impart a desired color, but have no nutritional or preservative function. Recently, the potential hazard to humans of ammonia- and ammonia-sulfite-process caramel colorings was raised, because they contain the by-product 4-methylimidazole, which is a potential carcinogen.1 The methylimidazole compounds are difficult to analyze due to their polar nature and low molecular weight. Traditional reversed phase techniques are...

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Improved Reproducibility and Reduced Sample Preparation Time for the HPLC Analysis of Aflatoxins in Raw Peanut Paste

    The US FDA and international regulatory agencies have set contamination levels for aflatoxins in animal feedstuffs. Since Aspergillus may infect commodities pre-harvest, during storage or during processing, monitoring for aflatoxins in associated agricultural commodities at all stages of production is requisite. Field screening methods exist that are adequate to estimate contamination levels for aflatoxins. When additional confirmation or quantification is desired, chromatographic laboratory analysis is often...

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Analytical and measuring instruments of shimadzu cannabis testing solutions

    Shimadzu`s Cannabis Testing Solutions cover a broad range of applications, from potency testing to pesticide screening. We supply the instruments, methods and experience necessary to get you up and running quickly. Shimadzu scientific instruments have been applied to natural products, food sciences, pharmaceuticals and environmental testing for decades. Whether you are a chromatography expert or a novice, our platforms and training ensure that your laboratory will generate quality results from a wide variety...

    By Shimadzu Scientific Instruments Inc based in Columbia, MARYLAND (USA).

  • Analysis of Methylimidazoles in Caramel Colored Carbonated Beverages

    Caramel colorings are used as additives in a broad range of food and beverage products to impart a desired color, but have no nutritional or preservative function. Recently, the potential hazard to humans of ammonia- and ammonia-sulfite-process caramel colorings was raised, because they contain the by-product 4-methylimidazole, which is a potential carcinogen.1 The methylimidazole compounds are difficult to analyze due to their polar nature and low molecular weight. Traditional reversed phase techniques are...

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Increased Pigment Removal with Analysis of Pesticides in Oranges

    An application was developed to demonstrate increased pigment removal for the analysis of pesticides, using Supel™ QuE Z-Sep/C18 QuEChERS and an Ascentis® Express C18 HPLC Column. The improved cleanup can decrease column and instrument fouling, leading to extended LC column lifetime and reduced instrument downtime.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Chromatography systems for pharmaceutical industries

    Dikma has over 15 years of R&D experience in chromatographic columns which have been widely used by pharmaceutical manufacturers.

    By Dikma Technologies Inc. based in Lake Forest, CALIFORNIA (USA).

  • Analysis of Herbicides in Drinking Water

    Herbicides are used throughout the world to combat the growth of unwanted plant life. These polar compounds are hydrophilic, so they may find their way into drinking water sources. While gas chromatography has historically been the preferred technique for analysis of herbicides in water samples, the use of LC-MS/MS for this application is gaining acceptance. This is due to the power of MS/MS to provide detailed identification of multiple analytes, but also in part due to the variety of HPLC stationary phases...

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Chromatography systems for food industries

    Food samples exhibit a challenge to analytical chemists to determine pesticide residues to meet food safety regulations in USA and EU. Therefore, more careful sample preparation is needed to successfully identify and quantify these compounds in food. Dikma offers SPE columns with different absorbent phases and QuEChERS method for sample pre-treament.

    By Dikma Technologies Inc. based in Lake Forest, CALIFORNIA (USA).

  • Determination of Additives in Beverages using Ascentis Express Columns

    Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications...

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Formaldehyde and Acetaldehyde Determination in Air Using Fully Automated On-Line Desorption and Analysis of DNPH Cartridges

    Airborne aldehydes and ketones are collected by passing air through a cartridge containing 2,4-dinitrophenylhydrazine (DNPH). Carbonyl compounds react with the DNPH to form hydrazones, which are immobilized on the cartridge. These compounds can be easily eluted from the cartridge with acetonitrile and analyzed by HPLC with UV detection. Traditionally, this analysis including the workup contains a series of manual steps, which can become time-consuming and could incur experimental error. Automating the extraction...

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Analysis of Isocyanates Using the ASSET™ EZ4-NCO Dry Sampler

    Isocyanates are used as a raw material to produce a number of products . Personal exposure can occur while the products made with isocyanates are being applied, or when the materials are removed by grinding or thermal degradation. Workers who are exposed to these compounds are at risk for respiratory disorders and asthma. The highly reactive nature of the isocyanate compounds and the low occupational exposure limits put high demands on both sampling and analytical techniques for monitoring of isocyanates in...

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Improved Reproducibility and Reduced Sample Preparation Time for the HPLC Analysis of Aflatoxins in Raw Peanut Paste

    The US FDA and international regulatory agencies have set contamination levels for aflatoxins in animal feedstuffs. Since Aspergillus may infect commodities pre-harvest, during storage or during processing, monitoring for aflatoxins in associated agricultural commodities at all stages of production is requisite. Field screening methods exist that are adequate to estimate contamination levels for aflatoxins. When additional confirmation or quantification is desired, chromatographic laboratory analysis is often...

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

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