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HPLC Applications

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    Analytical and measuring instruments of life science solutions industry

    Shimadzu`s Life Science solutions are designed to help accelerate life science research by integrating novel chemistry with innovative technology to enable life science researchers to automate and accelerate their laboratory workflows. Shimadzu brings together technologies and tools that help you successfully interrogate your biological sample. Whether it is a gel-based experiment for understanding protein expression, characterizing differences in metabolites in patient studies, or imaging tissue using mass spectrometry, Shimadzu can help you with your discovery, validation and translation challenges.

    By Shimadzu Scientific Instruments Inc based in Columbia, MARYLAND (USA).

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    Analytical and measuring instruments of shimadzu cannabis testing solutions

    Shimadzu`s Cannabis Testing Solutions cover a broad range of applications, from potency testing to pesticide screening. We supply the instruments, methods and experience necessary to get you up and running quickly. Shimadzu scientific instruments have been applied to natural products, food sciences, pharmaceuticals and environmental testing for decades. Whether you are a chromatography expert or a novice, our platforms and training ensure that your laboratory will generate quality results from a wide variety of cannabis samples. Our easy to use platforms save you time and money, maximizing the profits of your laboratory. Shimadzu can help you with your QA/QC testing, peak harvesting analysis and cannabis research.

    By Shimadzu Scientific Instruments Inc based in Columbia, MARYLAND (USA).

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    Analytical and measuring instruments of ceramics, metals, mining industry

    Geochemical Analysis: Applied geochemical analysis is of value to geologists, mining engineers, metallurgists, and geochemists and requires reliable instrumentation capable of accurate analysis in complex matrices. Whether looking for trace levels of precious metals, doing geochemical exploration on thousands of samples, or assaying concentrates or final product Shimadzu provides instrumentation that fits your needs.

    By Shimadzu Scientific Instruments Inc based in Columbia, MARYLAND (USA).

  • Chromatography systems for pharmaceutical industries

    Dikma has over 15 years of R&D experience in chromatographic columns which have been widely used by pharmaceutical manufacturers.

    By Dikma Technologies Inc. based in Lake Forest, CALIFORNIA (USA).

  • Analysis of Methylimidazoles in Caramel Colored Carbonated Beverages

    Caramel colorings are used as additives in a broad range of food and beverage products to impart a desired color, but have no nutritional or preservative function. Recently, the potential hazard to humans of ammonia- and ammonia-sulfite-process caramel colorings was raised, because they contain the by-product 4-methylimidazole, which is a potential carcinogen.1 The methylimidazole compounds are difficult to analyze due to their polar nature and low molecular weight. Traditional reversed phase techniques are unsuccessful in retaining these small polar compounds. Therefore, most HPLC methods utilize ion-exchange resins for analysis. Another common method involves GC analysis after the analytes first undergo a derivatization step. The purpose of the work shown in this article was to develop a simple and fast analytical method to determine the levels of 2-methylimidazole and 4-methylimidazole in caramel colored carbonated beverages.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Increased Pigment Removal with Analysis of Pesticides in Oranges

    An application was developed to demonstrate increased pigment removal for the analysis of pesticides, using Supel™ QuE Z-Sep/C18 QuEChERS and an Ascentis® Express C18 HPLC Column. The improved cleanup can decrease column and instrument fouling, leading to extended LC column lifetime and reduced instrument downtime.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Chromatography systems for food industries

    Food samples exhibit a challenge to analytical chemists to determine pesticide residues to meet food safety regulations in USA and EU. Therefore, more careful sample preparation is needed to successfully identify and quantify these compounds in food. Dikma offers SPE columns with different absorbent phases and QuEChERS method for sample pre-treament.

    By Dikma Technologies Inc. based in Lake Forest, CALIFORNIA (USA).

  • Determination of Additives in Beverages using Ascentis Express Columns

    Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications using Ascentis Express HPLC columns. Ascentis Express columns offer faster HPLC on any system. One benefit is their ability to produce the resolution, efficiency, and speed on conventional HPLC systems that is associated with the use of sub-2 micron columns on a UHPLC system, without generating high backpressure. Column chemistries (RP-Amide and HILIC) were selected for this article based on their enhanced performance with polar compounds in comparison to C18.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Formaldehyde and Acetaldehyde Determination in Air Using Fully Automated On-Line Desorption and Analysis of DNPH Cartridges

    Airborne aldehydes and ketones are collected by passing air through a cartridge containing 2,4-dinitrophenylhydrazine (DNPH). Carbonyl compounds react with the DNPH to form hydrazones, which are immobilized on the cartridge. These compounds can be easily eluted from the cartridge with acetonitrile and analyzed by HPLC with UV detection. Traditionally, this analysis including the workup contains a series of manual steps, which can become time-consuming and could incur experimental error. Automating the extraction of LpDNPH S10 cartridges and putting it in-line with the HPLC analysis will significantly reduce manual labor using this technique and this will improve reproducibility of the method by reducing potential experimental errors by the operator. The automation and unattended operation of the method leads to high throughput for determining airborne formaldehyde and acetaldehyde.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Improved Reproducibility and Reduced Sample Preparation Time for the HPLC Analysis of Aflatoxins in Raw Peanut Paste

    The US FDA and international regulatory agencies have set contamination levels for aflatoxins in animal feedstuffs. Since Aspergillus may infect commodities pre-harvest, during storage or during processing, monitoring for aflatoxins in associated agricultural commodities at all stages of production is requisite. Field screening methods exist that are adequate to estimate contamination levels for aflatoxins. When additional confirmation or quantification is desired, chromatographic laboratory analysis is often necessary. Preparation of matrix samples prior to chromatographic analysis typically requires extraction and purification. Commonly, immunoaffinity columns (IAC), which employ a multi-step bind and elute mechanism to concentrate and purify aflatoxins, are used to purify matrix samples for subsequent analysis. Solid phase extraction (SPE), an alternate method which may use interference removal, can also be employed.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

  • Analysis of Isocyanates Using the ASSET™ EZ4-NCO Dry Sampler

    Isocyanates are used as a raw material to produce a number of products . Personal exposure can occur while the products made with isocyanates are being applied, or when the materials are removed by grinding or thermal degradation. Workers who are exposed to these compounds are at risk for respiratory disorders and asthma. The highly reactive nature of the isocyanate compounds and the low occupational exposure limits put high demands on both sampling and analytical techniques for monitoring of isocyanates in air. We investigated the performance of the LC-MS and LC-MS-MS methods in the analysis of isocyanates using the new ASSET™ EZ4-NCO Dry Sampler. We found that the analytical method can successfully reach the quantitation limit for most isocyanates of 5 ng/mL in the final sample when LC-MS-MS analysis was used and the quantitation limit of 10 ng/mL when LC-MS analysis was used. All 11 compounds were well-resolved chromatographically using a 15 cm Ascentis Express C18 column.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

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