HPLC column Articles
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News on Air 2013, Volume 2: Products for Separation
Supelco® has developed the most extensive line of special purpose columns designed for industry specific applications; including environmental and industrial hygiene. These columns are manufactured to deliver high resolution, great analyte response, low bleed and long column life; allowing analysts to achieve the analytical performance they require. In this issue, you will find easy-to-read ...
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Microfiltration: Rocker VF12 Kit Saves Time and Money
Filtering organic solvents for applications such as HPLC buffer prep can be challenging in order to ensure chemical compatibility with filters (PVDF, Nylon, or PTFE), preserving longevity of an HPLC column, or containment of toxic fumes, but finding a suitable and comprehensive vacuum system is now easier than ever. Sterlitech is now offering a comprehensive benchtop vacuum system, the ...
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Separation of quercetin from a Ginkgo Biloba mother tincture using the PrepChrom C-700
The aim of this application note was to isolate a flavonol, quercetin, from a Ginkgo Biloba mother tincture. The prepurification of the mother tincture was performed on a reversed phase flash column by the means of dry loading. The target compound was identified by its characteristic UV-VIS spectra and the retention time determined by running a standard. Subsequently, the purification of the ...
By BUCHI
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HPLC prep - which filter fits your chromatography sample best?
Sample filtration protects your HPLC column and preserves data quality. Read our tips on filter and solvent selection and the use of tools to save time in HPLC prep, improve lab efficiency, and better your analytical chemistry techniques. Filtering samples prior to high-performance liquid chromatography (HPLC) analysis can help avoid frit clogging while maintaining data quality. Adding a ...
By Cytiva
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Chromatography Tips
No.1 Gas Chromatography Tips Our Founders and engineers of the company had accumulated more than 60 years experience in the research and development of HPLC instrumentation; HPLC column technology; mass spectrometer installation, training and services; chemical and biochemical separation, detection and quantification, Proteomics and cancer biomarker research and instrumentation, etc. Our goal ...
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News on Air 2013, Volume 1: Products for Sampling
For decades, Sigma-Aldrich has been a key supplier of innovative & high technology air monitoring products for various areas of gas/air sampling. Enclosed, you will find an overview on new and interesting devices to improve or simplify your air sampling. Supelco and Sigma-Aldrich offer a range of active and passive air sampling products, in addition to various analytical chemistry tools ...
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30-minute amino acid analysis of hydrolyzed samples
Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. No other tech¬nique has been shown to match the reproducibility and selectivity of ion-exchange analysis with post-column Ninhydrin detection. Nor is there a chromatography technique that provides as much information; the 570/440 nm signal ratio for each amino acid is a ...
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The Clog Blog
Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...
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o-Phthalaldehyde Reagent
Introduction Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. At a pH>9 and ambient temperature, reaction is generally complete within 30 seconds. The products of this reaction, 1-alkyl-2-alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm. The reagents described ...
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ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines
The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work ...
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Aflatoxin & ochratoxin sample preparation columns
Simultaneous Analysis of Aflatoxins and Ochratoxin A - Sample Preparation Aflatoxins and Ochratoxin A are produced by fungi, e.g. Aspergillus and Penicillium species. Therefore both toxin types are found together in many foods and animal feeds, e.g. cereals. Thus it is appropriate to analyze one extract for the occurrence of both mycotoxins in a single step. A significant assistance is the ...
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Pinnacle PCX Carbamate Testing at Nebraska Health
Beta Site Evaluation of the EPA Carbamate in Drinking Water Method 531.1 with Pickering Laboratories Pinnacle PCX Integrated LC/Post-Column Derivatization Instrument. The Nebraska DHHS Public Health Environmental Laboratory conducts environmental testing for the state of Nebraska. It employs mandated U.S. Environmental Protection Agency (EPA) methods for the determination of regulated substances ...
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Find out the principles of ELSD and the gas delivery solutions for ELSD from a nitrogen gas generator
ELSD is one of the most common detectors used with HPLC and is preferred to other detection methods for analyses such as carbohydrate, natural sugars and polymer analysis as well as being used for a wide range of other applications. As a mass-detector, ELSD has advantages over some other methods of detection such as UV detection that require analytes to contain chromophores and it is unaffected ...
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Find out the principles of ELSD and the gas delivery solutions for ELSD from a nitrogen gas generator
ELSD is one of the most common detectors used with HPLC and is preferred to other detection methods for analyses such as carbohydrate, natural sugars and polymer analysis as well as being used for a wide range of other applications. As a mass-detector, ELSD has advantages over some other methods of detection such as UV detection that require analytes to contain chromophores and it is unaffected ...
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Analysis of Voglibose in pharmace utical formulat ions by HPLC with post-column derivat izat ion
Voglibose is an Alpha-Glucosidase inhibitor widely used for the treatment of diabetes. Alpha-glucosidase inhibitors are agents that delay the glucose absorption at the intestinal level and thereby prevent sudden surge of glucose after a meal. Vogilbose is the safest and most effective drug of its class. Since Vogibose has no UV chromophore, post-column derivatization is employed to produce a ...
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Sulfonamide artificial sweeteners in food products analysis of acesulfame, saccharin and cyclamate by HPLC with post-column derivatization
Nonnutritive sweeteners are widely used in foods and beverages. Since some studies raised questions about long-term safety of these compounds, their concentration in food products is regulated. Among the artificial sweeteners used worldwide Saccharin and Cyclamate cause most controversy and their use is restricted in many countries, including the US. Liquid chromatography is a method of choice ...
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Tips to maintain your HPLC & UHPLC systems and columns
Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your LC columns Proper storage of HPLC ...
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Bromate analysis analysis of bromate in drinking water by hplc and post-column derivatization
Bromate is a disinfection by-product that is formed when Ozone reacts with naturally occurring Bromide in drinking water. Bromate is a known animal carcinogen and has also been listed as a group 2B toxin: probable human carcinogen. The U.S. EPA Method 300.1 employs conductivity as the means of detection which works well for most anions. However, the method is nonspecific and coeluting ...
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Expanded HPLC Method for N-Methyl Carbamates post-column analysis of pesticides in food and potable water samples
Carbamate pesticides are widely used around the world to protect crops. In addition, they are used as biocides for industrial or other applications and in household products. Though carbamates are biodegradable compounds and bioaccumulation usually happens only to a low extend it is important to monitor produce to make sure enough time has elapsed between harvest and applying pesticides. Also, ...
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Application of HPLC Technology in Drug Detection
What is HPLC technology? High performance liquid chromatography (HPLC) is also called "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography" and so on. High performance liquid chromatography is an important branch of chromatography. It uses liquid as the mobile phase, and uses a high-pressure injection system to pump single solvents with ...
By BOC Sciences
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