used HPLC Articles
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Analysis of Bisphenol A on Ascentis Express RP-Amide
Analysis of Bisphenol A and Related Compounds on Ascentis™ Express RP-Amide This application report shows the efficient separation of Bisphenol A, Dimethyl Bisphenol A, and Nonylphenol, via HPLC using an Ascentis Express RP-Amide column, under gradient conditions and with UV detection. ...
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Comparative study for brain delivery of tacrine using polysorbate 80-coated poly(butylcyanoacrylate) and pegylated-poly(butylcyanoacrylate) nanoparticles
Many studies indicated that polysorbate (PS) 80-coated polybutylcyanoacrylate (PBCA) nanoparticles can deliver drugs that poorly penetrate BBB to the brain. Also, the polyethylene glycolated-polybutylcyanoacrylate (PEG-PBCA) nanoparticles have a good safety profiles and sustained drug release. The purpose of this study is to compare between the ability of both PS80-coated PBCA nanoparticles and ...
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Development of a Cleaning Validation Method for MICRO® A07 Citric Acid Cleaner Using HPLC-CAD
Abstract A high performance liquid chromatography (HPLC) method with a Corona Charged Aerosol detector for the cleaning validation of MICRO* A07 Citric Acid Cleaner residue has been developed. The pooled average repeatability of 3 replicates at 6 concentration levels expressed as RSD was 1.3 % while the pooled average intermediate precision (intra-day repeatability in 3 days) of 3 replicates at 6 ...
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Rapid on‐site separation of as(III) and as(V) in waters using a disposable thiol‐modified sand cartridge
The rapid redox transformation of arsenic (As) species in waters presents a great environmental challenge in accurate determination of its concentration and toxicity. The motivation for our study is therefore to develop a method for rapid on‐site separation of As(V) and As(III) in various aqueous matrices. We synthesized a thiol‐modified sand (T‐sand), which selectively removed As(III) but did ...
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Low Level Determination of Polynuclear Aromatic Hydrocarbons in Environmental Samples, Using GC/MS
Low level analysis of PAHs is required to attain various clean-up limits for environmental regulations. The ability to maximize GC/MS instrument conditions to meet lower reporting levels results in a more efficient and reliable determination of these analytes. The following analysis provides the necessary sensitivity and selectivity. The procedure requires that the parameters for GC as well as MS ...
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Cannabis quality tests now much faster thanks to HPLC
A new method of measuring phytocannabinoids has been developed and HPLC has been found to hold the key to faster measurements times. Read on to learn more and to discover our nitrogen generators for HPLC – ELSD. Researchers from the University of British Columbia have created a new method of measuring phytocannabinoids which is more than twice as quick as traditional methods. ...
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Arsenic, antimony and chromium speciation using HPLC-ICP-MS in selected river ecosystems of Upper Silesia, Poland – a preliminary study and validation of methodology
Upper Silesia is the most transformed by humans of the Polish territories. As a result of mining, urbanization and other industrial activities, irreversible changes have taken place in the environment. In this paper, the method of collecting, preserving, storing and preparing samples from four Silesian rivers was optimized. Silesian sediment and river water samples are a challenge for the ...
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The development of cation-exchange chromatography in free amino acids analysis
Although it was not called a liquid chromatograph, the first such instrument was introduced in late 1959 by Beckman Instruments and was designated the M.S. Amino Acid Analyzer in honour of Moore and Stein. Inject valves had yet to be invented, the buffer switching valves were glass stopcocks and the column temperature was controlled by circulating water baths. Two positive displacement pumps ...
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What can I use to filter my mobile phase?
If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed very well in a blog post written by one of my colleagues: ...
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GC-MS/MS analysis of PAH and PCB in environmental samples
The polychlorinated biphenyls (PCB) and polyaromatic hydrocarbons (PAH) are among the most commonly analysed compound classes in environmental analysis. This article demonstrates how hydrogen carrier gas can be used in place of helium for GC-MS/MS analysis of these compounds. Analysis of polyaromatic hydrocarbons (PAH) is typically conducted using HPLC with UV- and fluorescence detection. ...
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A bridge with our customers - Prominence-i (i-Series) - Case Study
Anthem Biosciences, which is ranked among the top 5 leading Contract Research Organisations (CROs) in India, is emerging as a global leader in the domain of Integrated Pharmaceuticals business. Anthem, which calls itself a Contract Research and Innovation Service Provider (CRISP), offers a whole gamut of services dedicated to enabling and sustaining global research efforts in the discovery of new ...
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Sample collection and analysis of carbonyls - Improve detection of trace level contaminants in air
Improve Sampling and Detection of Carbonyls in Air Leverage a Wide Range of Samplers and Accessories Achieve sensitive and reliable results with air sampling media for a wide range of applications in various configurations for solvent desorption, thermal desorption, passive and whole air sampling from Supelco. Ensure low background for carbonyl sampling with products that are produced to the ...
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Application of HPLC Technology in Drug Detection
What is HPLC technology? High performance liquid chromatography (HPLC) is also called "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography" and so on. High performance liquid chromatography is an important branch of chromatography. It uses liquid as the mobile phase, and uses a high-pressure injection system to pump single solvents with ...
By BOC Sciences
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Purification of natural products - Case Study
“Due to its versatility, the PrepChrom C-700 is a valuable instrument in our natural product isolation based research group, where preparative techniques such as column chromatography, flash chromatography and preparative HPLC are used on a daily basis.” Customer name Prof. Dr. Timo Niedermeyer Company / Institution University of Tübingen, Germany Product lines Preparative ...
By BUCHI
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Find out the principles of ELSD and the gas delivery solutions for ELSD from a nitrogen gas generator
ELSD is one of the most common detectors used with HPLC and is preferred to other detection methods for analyses such as carbohydrate, natural sugars and polymer analysis as well as being used for a wide range of other applications. As a mass-detector, ELSD has advantages over some other methods of detection such as UV detection that require analytes to contain chromophores and it is unaffected ...
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Find out the principles of ELSD and the gas delivery solutions for ELSD from a nitrogen gas generator
ELSD is one of the most common detectors used with HPLC and is preferred to other detection methods for analyses such as carbohydrate, natural sugars and polymer analysis as well as being used for a wide range of other applications. As a mass-detector, ELSD has advantages over some other methods of detection such as UV detection that require analytes to contain chromophores and it is unaffected ...
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Cell Penetrating Peptides: Definition and Synthesis
What are cell penetrating peptides? Cell Penetrating Peptides (CPPs) are short peptides that have the ability to cross biological membranes and enter cells. They are able to traverse the cell membrane by directly translocating across the lipid bilayer or by exploiting endocytic pathways. CPPs are generally composed of 5-30 amino acids and are known for their high cell-penetrating efficiency and ...
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GC-MS used by Forensic experts in Mumbai to detect intoxicants and help solve crime
Forensic experts in Mumbai have solved the problem of analysing old viscera samples for intoxicants by using GC-MS. The Directorate of Forensic Science Laboratories (FSL) recently commented on a case they are working on involving the death of a man in Mumbai. In this case there is ambiguity as to whether or not the deceased man had intoxicants, such as drugs or alcohol, in his system immediately ...
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HPLC prep - which filter fits your chromatography sample best?
Sample filtration protects your HPLC column and preserves data quality. Read our tips on filter and solvent selection and the use of tools to save time in HPLC prep, improve lab efficiency, and better your analytical chemistry techniques. Filtering samples prior to high-performance liquid chromatography (HPLC) analysis can help avoid frit clogging while maintaining data quality. Adding a ...
By Cytiva
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o-Phthalaldehyde Reagent
Introduction Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. At a pH>9 and ambient temperature, reaction is generally complete within 30 seconds. The products of this reaction, 1-alkyl-2-alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm. The reagents described ...
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