HPLC Articles
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Amino acid supplementation for enhancing recombinant protein production in E. coli
Most recombinant protein production processes with microbial cell lines like E. coli have traditionally used complex media made up of tryptone and yeast extract to provide the necessary nutrients for cell health and productivity. However, variations in the complex media and potential impurities in different batches can cause serious problems during a bioprocess. For example, amino acid starvation ...
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Vaccine Quality Control: Total Protein Determination by TN Analysis
Vaccine quality: Determine total protein content using TN analysis TN stands for the total nitrogen. The sum parameter is easy to measure and allows safe and reliable conclusions about the total protein content in a pharmaceutical aqueous solution. The total protein content is an important quality parameter in pharmaceutical vaccine production. This article explains how you can determine the ...
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Determination of Aromatic Hydrocarbons in Diesel Fuel by LC-RID According to ASTM D6591
The test method presented here is applicable to the determination of mono-, di-, and tri-plus aromatic hydrocarbon (MAH, DAH, and T+AH, respectively) content in diesel fuels and middle distillates with boiling points in the range of 150° to 400° C in accordance with ASTM D6591. The accurate determination of the aromatic content of fuels is critical to assessing their quality and combustion ...
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Analysis of Tetrahydrocannabinol Vape Oils Using Pyroprobe by Thermal Extraction
Abstract This application note demonstrates a multi-step thermal extraction study on Tetrahydrocannabinol (THC) vape oils. Introduction E-cigarettes have gained popularity in recent years, in conjunction with the legalization of marijuana in many U.S. states. Authentic THC vape oils are not easily distinguishable from the counterfeits, which are poorly regulated and can cause severe lung ...
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Predicting the Toxicity of Oil-Shale Industry Wastewaters by its Phenolic Composition
Abstract : The chemical composition and toxicity of five phenolic wastewaters samples collected from the Kohlta-Järve (Estonia) oil-shale industry region were analysed. The total phenolic contents (HPLC data) of these samples ranged from 0.7 mg/l to 195 mg/l. A total of 11 phenol (up to 84 mg/l) and p-cresol (up to 74 mg/l). Artificial phenolic mixtures were also composed, to mimic the ...
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The Toxicity of Cyanobacterial Blooms as Determined by Microbiotests and Mouse Assays
Abstract : Cyanobactrial blooms are common throughout the world in eutrophic water bodies. Cyanobacterial hepato- and neurotoxins represent a health threat for humans as well as for animals in drinking water supplies. Mouse bioassays have been used extensively to detect cyanobacterial blooms toxicity but the use of warm-blooded vertebrates is limited to specialised laboratories and poses ethical ...
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Syringe Pump Applications In Bioscience & Biochemistry
In an era when biochemical research goes down to a single cell or even a single molecule level, there is a constant demand for ever more precise equipment. Considering that an accurate administration of small amounts of liquid is an imperative in any contemporary biochemical laboratory, the past few decades saw a vivid evolution of syringe pumps, carefully constructed and adapted for targeted ...
By Chemyx Inc
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Purification of natural products - Case Study
“Due to its versatility, the PrepChrom C-700 is a valuable instrument in our natural product isolation based research group, where preparative techniques such as column chromatography, flash chromatography and preparative HPLC are used on a daily basis.” Customer name Prof. Dr. Timo Niedermeyer Company / Institution University of Tübingen, Germany Product lines Preparative ...
By BUCHI
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Himachal Pradesh University, Shimla, India - Case Study
“With Encapsulator B-390 we could effi ciently entrap whole microbial resting cells and free enzymes in different matrices which can be used for various biotransformation reactions for the synthesis of various commodity chemicals and products of immense industrial and pharmaceutical signifi cance.” Company / Institution Himachal Pradesh University, Shimla, India Product lines ...
By BUCHI
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Federal association, France - Case Study
“Syncore Analyst P-12 in combination with the Vacuum Pump V-700 Advanced and the Recirculating Chiller, has really made our daily work more easy. We don’t have to check the flasks all the time to avoid concentrating to dryness, and we protect the environment, which is one of our major concerns!” Company / Institution Federal association, France Product lines Parallel ...
By BUCHI
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Stainless Steel Capillary Tubes
Product descriptionStainless Steel Capillary Tube Description:Whether you need to exchange device tubing as a section of your troubleshooting or want to minimize the dwell extent of your system as you pass to narrower columns. As one of the most professional stainless steel capillary tube suppliers in China, WENZHOU HUASHANG STEEL CO., LTD. has the first-rate tubing in the lengths. Tubing is ...
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WHY PEEK MATERIAL IS WIDELY USED IN SEALING TERMINAL & CONNECTOR APPLICATION IN OIL&GAS FIELD?
PEEK connector, we also call it PEEK sealing terminal or HTHP connector, just as its name implies which the connector is made from PEEK material combining with metal contact and using glass sintered technology sealing PEEk material & contact so it has perfect air leakage performance. In wellhead, gate valve requires to PEEK terminal for sealing, or tubing head spool for connect gauge or ...
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Introducing Sterlitech’s Expanded Range of Syringe Filters
Sterlitech Syringe Filters play important roles in the purification processes inside laboratories and out in remote operations—applying to sample preparation for HPLC, pharmaceutical dissolution testing, and environmental monitoring, to name a few. Industries and applications that use syringe filters continue to transform and expand, with the market expected to grow at 3.3%(1) for the ...
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WHY PEEK MATERIAL IS WIDELY USED IN SEALING TERMINAL & CONNECTOR APPLICATION IN OIL&GAS FIELD?
PEEK connector, we also call it PEEK sealing terminal or HTHP connector, just as its name implies which the connector is made from PEEK material combining with metal contact and using glass sintered technology sealing PEEk material & contact so it has perfect air leakage performance. In wellhead, gate valve requires to PEEK terminal for sealing, or tubing head spool for connect gauge or ...
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Hard to Find Filter Configurations
Announcing New Hard to Find Filters Filters have been the go-to method for sampling air in determining particulate contamination. Before I get into the “hard to find” filters, let us talk about filtration. The ideal filter should: be able to capture “everything” down to the nanometer; never plug; be free of the substance that you are measuring, down to nanograms; ...
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Cannabis quality tests now much faster thanks to HPLC
A new method of measuring phytocannabinoids has been developed and HPLC has been found to hold the key to faster measurements times. Read on to learn more and to discover our nitrogen generators for HPLC – ELSD. Researchers from the University of British Columbia have created a new method of measuring phytocannabinoids which is more than twice as quick as traditional methods. ...
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Sample collection and analysis of carbonyls - Improve detection of trace level contaminants in air
Improve Sampling and Detection of Carbonyls in Air Leverage a Wide Range of Samplers and Accessories Achieve sensitive and reliable results with air sampling media for a wide range of applications in various configurations for solvent desorption, thermal desorption, passive and whole air sampling from Supelco. Ensure low background for carbonyl sampling with products that are produced to the ...
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What is electrochemical detection and what are its applications?
Electrochemical detection (ECD) for HPLC or UHPLC / UPLC is an extremely selective and sensitive detection technique that is applied in several analyses such as the neurotransmitters dopamine, serotonin and noradrenalin, or analytes such as 5HIAA, catecholamines and metanephrines. ECD has an enormous linear dynamic range of more than 6 orders of magnitude. This means that concentrations can be ...
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GC-MS used by Forensic experts in Mumbai to detect intoxicants and help solve crime
Forensic experts in Mumbai have solved the problem of analysing old viscera samples for intoxicants by using GC-MS. The Directorate of Forensic Science Laboratories (FSL) recently commented on a case they are working on involving the death of a man in Mumbai. In this case there is ambiguity as to whether or not the deceased man had intoxicants, such as drugs or alcohol, in his system immediately ...
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Syringe Pumps vs Peristaltic Pumps - Case Study
Introduction Different applications require a pump, among all the options peristaltic and syringe pumps, are the most suitable. However, selection of the best is always a disjunctive. In general terms, laboratory and industrial process use peristaltic pumps. A user needs to know more about the systems and proper comparison of these technologies. In some cases is difficult to understand what are ...
By Chemyx Inc
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