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Sample Dilution Applications

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    Determination of Additives in Beverages using Ascentis Express Columns

    Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications using Ascentis Express HPLC columns. Ascentis Express columns offer faster HPLC on any system. One benefit is their ability to produce the resolution, efficiency, and speed on conventional HPLC systems that is associated with the use of sub-2 micron columns on a UHPLC system, without generating high backpressure. Column chemistries (RP-Amide and HILIC) were selected for this article based on their enhanced performance with polar compounds in comparison to C18.

    By Merck KGaA, Darmstadt, Germany based in Darmstadt, GERMANY.

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    Determination of acetic acid (acetate anions) in natural, drinking, and waste water

    Introduction

    The method is used for the determination of acetic acid (as acetate ions) in the samples of natural water, drinking water, and waste water by capillary electrophoresis.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

  • APHA Color #3020

    Measurement of APHA color either on-line or in a laboratory setting using a photometer. Background: APHA is sometimes referred to as the Platinum Cobalt (Pt/Co) or Hazen scale. Also referred to as a “yellowness index”, the APHA color scale is a common method of comparison of the intensity of yellow-tinted samples to assess the quality of liquids that are clear to yellowish in color. Originally developed to determine the purity of municipal water supplies, it is now used as a metric for purity in the water, chemical, oil, plastics, and pharmaceutical industries. APHA color quantifies the appearance of trace amounts of yellowness, which is a visual indicator of product degradation due to exposure to light or heat; the presence of impurities and negative effects of processing. As such APHA color is often used as a product release specification. APHA is a single number yellowness index. The units are based on a dilution of a 500ppm solution of PtCo.

    By Guided Wave, Inc. based in Rancho Cordova, CALIFORNIA (USA).

  • Determination of Additives in Beverages using Ascentis Express Columns

    Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable. In this article we present two beverage applications using Ascentis Express HPLC columns. Ascentis Express columns offer faster HPLC on any system. One benefit is their ability to produce the resolution, efficiency, and speed on conventional HPLC systems that is associated with the use of sub-2 micron columns on a UHPLC system, without generating high backpressure. Column chemistries (RP-Amide and HILIC) were selected for this article based on their enhanced performance with polar compounds in comparison to C18.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

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