bacteria sampling Articles
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Bacteria Preservation with Froilabo
What is Bacteria Preservation?Bacteria preservation is the process of treating and storing bacteria cultures for future use. Preservation of bacteria optimises cell viability for use at a later date, for example in research or diagnostics months or even years after sample collection.Why is Bacteria Preservation Important?If bacteria cultures were stored at room temperature, the cells would ...
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Accuracy of the H2S test: a systematic review of the influence of bacterial density and sample volume
The presence/absence hydrogen sulphide test (P/A H2S) is widely used as a low-cost alternative faecal indicator test in remote and resource-poor settings. The aim of the paper is to assess how bacterial density and sample volume affect its accuracy. Based on a systematic search, we identified studies that tested water samples (n = 2,034) using both the P/A H2S test and recognised tests ...
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Detection of Bacillus anthracis spores in postal and environmental samples in cases of suspected bioterrorism
From October 2001 to the end of 2004, a series of postal samples containing material suspected of containing Bacillus anthracis spores were examined. Environmental samples from dwelling places of some addressees and laboratory areas, including equipment, were also examined. Classical culture methods for isolation and identification were slightly modified and conformed to environmental samples. ...
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CASE STUDY - K5 Purefecta cartridge tested by Mycometer
The Kinetico K5 drinking water station with Purefecta cartridge clearly passed the test when examined by the Bactiquant®-water method (US-EPA, verified technology) developed by Mycometer. The method is based on a highly sensitive fluorescence technology which is used to detect and quantify an enzyme activity in bacteria. The fluorescence produced is directly proportional to the content of ...
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Effect of daylight on regrowth of bacteria in anaerobically digested sludge
This study investigated the regrowth of total coliform, Salmonella, and Clostridium perfringens in anaerobically digested sludge after centrifuge dewatering in the presence and absence of daylight. Sludge cake and centrate samples were collected from a treatment plant, and half of the samples was stored in daylight and the other half was stored in dark for three weeks. The bacteria levels in the ...
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Legionella Bacteria Water Sampling Methodology
This blog delves into systematic approaches for Legionella water testing, offering valuable insights into best practices and recommendations to ensure the safety of your ...
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Antibiotic-resistant bacteria in the Hudson River Estuary linked to wet weather sewage contamination
Heterotrophic bacteria resistant to tetracycline and ampicillin were assessed in waterways of the New York City metropolitan area using culture-dependent approaches and 16S rRNA gene sequence analysis of resultant isolates. Resistant microbes were detected at all 10 sampling sites in monthly research cruises on the lower Hudson River Estuary (HRE), with highest concentrations detected at ...
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Evaluation of pcr primer selectivity and phylogenetic specificity by using amplification of 16s rrna genes from betaproteobacterial ammonia-oxidizing bacteria in environmental samples
The effect of primer specificity for studying the diversity of ammonia-oxidizing betaproteobacteria (βAOB) was evaluated. βAOB represent a group of phylogenetically related organisms for which the 16S rRNA gene approach is especially suitable. We used experimental comparisons of primer performance with water samples, together with an in silico analysis of published sequences and a ...
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Quantification and identification of aerobic bacteria in holy water samples from a German environment
Despite its frequent use in many religious institutions, the microbiological quality of holy water is clearly underinvestigated. We analyzed the microbial load of 54 holy water samples, repeatedly taken in five Roman Catholic churches in the greater area of Villingen-Schwenningen, Germany, by means of aerobic colony counting and Matrix-Assisted Laser Desorption/Ionization (MALDI) Biotyping of ...
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Indoor air quality problems – If it isn’t Mold, What is it?
Why are you experiencing indoor air quality problems? There are a number of employees in our office who have itchy, watery eyes, nasal congestion, headaches, lethargy, and trouble concentrating. Most of the affected folks report they feel better at home and worse after they’ve been in the office for about an hour. We had the office tested for mold but nothing remarkable was found. ...
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Influencing factors in the occurrence of injured coliforms in the drinking water distribution system in the city of La Plata, Argentina
The objective of this study was to evaluate the influencing factors in the occurrence of coliforms in the drinking water in La Plata (Argentina) from June 1999 to June 2001. A total of 180 samples were collected from Rio de La Plata (102 samples) and Puelche Aquifer (78 samples); 45 samples were collected for each of the four seasons. The membrane filter procedure was used for isolating bacteria, ...
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Population dynamics of filamentous bacteria identified in Polish full-scale wastewater treatment plants with nutrients removal
A comprehensive study of the identity and population dynamics of filamentous bacteria in five Polish full-scale municipal wastewater treatment plants (WWTPs) with nutrients removal had been carried out for 2 years. A quantitative culture-independent, molecular method – fluorescence in situ hybridization – was applied to evaluate the structure of different filamentous bacteria ...
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HOW TO PROPERLY COMPARE ATP TESTING AND CULTURE?
ATP testing and bacterial culture on solid agar plates are two completely different techniques. While culture method only measures culturable bacteria, i.e., the ones able to grow on a given media, ATP testing measures the quantity of ATP in a sample. As this molecule is produced and found in all living bacteria, ATP testing measures all the bacteria, culturable or not. This ...
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How CRISPR Works
Back in 2011, Jennifer Doudna, a biochemist and molecular biologist at the University of California, Berkeley, and Emmanuelle Charpentier, now at the Max Planck Institute for Infection Biology in Germany, grew intrigued by the way bacteria use a molecular system known as CRISPR-Cas9 to respond to viral attacks. For years, bacteria were assumed to be primitive creatures with rudimentary immune ...
By Ensia
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Effects of ultraviolet light disinfection on tetracycline-resistant bacteria in wastewater effluents
The ubiquitous use of antibiotics has led to an increasing number of antibiotic-resistant bacterial strains, including strains that are multidrug-resistant, pathogenic, or both. There is also evidence to suggest that antibiotic resistance genes (ARGs) spread to the environment, humans, and animals through wastewater effluents. The overall objective of this study was to investigate the effect of ...
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Microbiological quality of ice and ice machines used in food establishments
The ice used in the food industry has to be safe and the water used in ice production should have the quality of drinking water. The consumption of contaminated ice directly or indirectly may be a vehicle for transmission of pathogenic bacteria to humans producing outbreaks of gastrointestinal diseases. The objective of this study was to monitor the microbiological quality of ice, the water ...
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Ultrasonic impact on bacterial population in sewage sample
Nine samples of sewage sludge from different paper mills of New Delhi were treated with ultrasonic waves of two different frequencies, viz., 35 kHz and 130 kHz for different time periods (5, 10, 20 and 30 min). Treated sewage sludge was then evaluated with different parameters, viz., Chemical Oxygen Demand(COD), Biochemical Oxygen Demand (BOD), Total Nitrogen, Total Phosphorus, Total Ammonia and ...
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Evaluation of Nature’s Broom Plus for remediation of used motor oil and hydraulic fluid - Case study
Summary: In a nine week study, a bio-based oil absorbent, Nature's Broom Plus, was evaluated for its ability to stimulate the degradation of petroleum products in used motor oil and hydraulic fluid. There were three treatments: 1) controls containing only Nature's Broom Plus and de-ionized water, 2) Nature's Broom Plus contaminated with - 20,000 ppm of Havoline motor oil and de-ionized water and ...
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Removal of bacteria, protozoa and viruses through a multiple-barrier household water disinfection system
Municipal water disinfection systems in some areas are not always able to meet water consumer needs, such as ensuring distributed water quality, because household water management can be a contributing factor in water re-contamination. This fact is related to the storage options that are common in places where water is scarce or is distributed over limited time periods. The aim of this study is ...
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Development and validation of a FISH-based method for the detection and quantification of
E. coli and coliform bacteria in water samplesMonitoring of microbiological contaminants in water supplies requires fast and sensitive methods for the specific detection of indicator organisms or pathogens. We developed a protocol for the simultaneous detection of E. coli and coliform bacteria based on the Fluorescence in situ Hybridization (FISH) technology. This protocol consists of two approaches. The first allows the direct detection of ...
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