Pharmaceutical research starts with lead molecule identification from a natural or artificial model. In either case, the DMPK or animal toxicology models analysis requires the homogenization of tissues.
Hard tissues such as those from tumors can be particularly difficult to homogenize, and animal models are often used in cancer research. Precellys homogenizer is perfectly adapted to applications of this kind.
A number of methods have been reported for the measurement of calcium in biological materials by flame photometry or titration. This method employs trichloroacetic acid (TCA) to precipitate the proteins and has been found to be adequate to extract all of the calcium from tissue samples. Sodium, potassium and phosphate caused no significant interference and calcium concentration in the range of 0 to 2 µg/ml is readily determined.