UPLC Articles
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High Performance Liquid Chromatography (HPLC) and Pharmaceutical Analysis
High performance liquid chromatography (HPLC) has become an essential technique in pharmaceutical analysis for the separation, identification, and quantification of pharmaceuticals. Due to its high resolution and sensitivity, it is used for testing drug purity, monitoring degradation, studying pharmacokinetics, and examining drug stability. With recent advances in instrumentation, the technique ...
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Amino acid supplementation for enhancing recombinant protein production in E. coli
Most recombinant protein production processes with microbial cell lines like E. coli have traditionally used complex media made up of tryptone and yeast extract to provide the necessary nutrients for cell health and productivity. However, variations in the complex media and potential impurities in different batches can cause serious problems during a bioprocess. For example, amino acid starvation ...
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Impact of Cell Culture Media Additives on IgG Glycosylation Produced in Chinese Hamster Ovary Cells
IQVIA recently released a comprehensive overview of the US pharmaceutical market. The report highlighted that despite recent approvals of biosimilars, they are used less than one-third of the time when available. Additionally, at most recent conferences, there have been several dedicated sessions discussing the challenges developing, manufacturing and commercializing biosimilars. Dahodwala and ...
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Stainless Steel Capillary Tubes
Product descriptionStainless Steel Capillary Tube Description:Whether you need to exchange device tubing as a section of your troubleshooting or want to minimize the dwell extent of your system as you pass to narrower columns. As one of the most professional stainless steel capillary tube suppliers in China, WENZHOU HUASHANG STEEL CO., LTD. has the first-rate tubing in the lengths. Tubing is ...
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What is electrochemical detection and what are its applications?
Electrochemical detection (ECD) for HPLC or UHPLC / UPLC is an extremely selective and sensitive detection technique that is applied in several analyses such as the neurotransmitters dopamine, serotonin and noradrenalin, or analytes such as 5HIAA, catecholamines and metanephrines. ECD has an enormous linear dynamic range of more than 6 orders of magnitude. This means that concentrations can be ...
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Identification and risk assessment of human and veterinary antibiotics in the wastewater treatment plants and the adjacent sea in Tunisia
In the following study, we came up with and validated a prompt, sensitive and precise method for the simultaneous determination of 56 antimicrobial drugs (tetracyclines, sulfonamides, β-lactams, macrolides and quinolones) using the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This method was implemented with success to determine antibiotics in samples ...
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Photodegradation of the benzothiostrobin in solution and on soil and glass surface
The photolytic characteristics of benzothiostrobin were investigated in solution and on soil and glass surface. The main influence factors such as initial concentration, organic solvent and the aqueous environmental substances, including NO2–, NO3–, Fe2+, Fe3+, H2O2 and turbidity were investigated. Three photodegradation products of benzothiostrobin were identified by ultra-performance liquid ...
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How Water Treatment Plants Protect Fish from Disruptive Synthetic Hormones
Scottish Water provides 1.34 billion liters of drinking water every day and takes away 847 million liters of waste water daily. Answerable to both the Scottish Parliament and the people of Scotland, Scottish Water recognizes that innovation is a key enabler for them to deliver sustainable, high-quality water treatment services. One of the many compounds they monitor for is the drug class of ...
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Building a Perfect Purification Platform
Dart NeuroScience is one of the largest privately owned biotech companies in San Diego with around 250 employees – and we have an interesting focus: memory. More specifically, our aim is to develop new technologies and therapies that can maintain or improve memory function and cognitive vitality. Much of our research is built on the work of Tim Tully, who was a professor and Head of the ...
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Ultra-Pure Proof with Mass Detection
Before joining the newly formed Reach Separations in February 2012, I spent five years at AstraZeneca in analysis and purification (latterly as group lead in reversed phase and chiral purification). This experience gives me a good perspective of both the needs of “big pharma” and, more recently, the needs of a specialist separation solution provider. My time at AstraZeneca allowed me ...
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Webinar Recap: “Developing, Testing, and Troubleshooting Chromatography Method Transfer”
Hi, I’m Tom Wheat, Principal Scientist at Waters, and I recently hosted a webinar entitled, “Developing, Testing and Troubleshooting Chromatography Method Transfer.” Over the course of the webinar, we discussed the transfer of chromatographic methods that occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one ...
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Get Empowered: Quickstart Interface - Tip #1
Welcome back to Get Empowered! My tip-of-the-week blog for Empower Software kicks-off by taking a closer look at the Empower QuickStart Interface. There is so much to cover in this series, so I have broken it up in to three tips. Tip #1: An Overview Tip #2: Interactive Help (‘Show me’) Tip #3: Working with Projects and Systems Let’s get started! Tip #1: An Overview The ...
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Advancing Omics Research and Reducing Bias with New MS Technology for Data-Independent Acquisition Experiments
Targeted LC-MS based assays are increasingly applied in the post-discovery Omics area with an emphasis on validation, the first of many phases in translational research; and in studies that are aimed at gaining the understanding of biological systems in terms of drug development and treatment. Understanding the context of analytical results is driving current research, and therefore driving the ...
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Webinar Highlights: Microfluidics for macromolecules
Hi, I’m Jim Murphy, Principal Consulting Chemist at Waters, and I recently collaborated with Lucinda (Cohen) Hittle to present an exciting webinar on the use of microflow chromatography for macromolecules. Lucinda, a Director of Discovery Bioanalytics at Merck, presented an overview of the use of LC-MS for peptide analysis along with various case studies for in vitro and in vivo studies ...
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Tips to maintain your HPLC & UHPLC systems and columns
Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your LC columns Proper storage of HPLC ...
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Is Microflow LC a Reality or Perception?
Microflow or nanoflow chromatography has been used mostly in the academic and fundamental research environment in the past for its various advantages over analytical liquid chromatography, but rarely in the industrial settings. This is most likely because many of us believe it is a pain-in-the-neck technique to perform! But is this a reality or just a perception? When I was in graduate school ...
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Chivas Brothers Limited - Case Study
Name: Alan Rettie Job title: Development Chemist Company: Chivas Brothers Limited (UK) Chivas Brothers, the Scotch whisky subsidiary of French premium spirit conglomerate Pernod Ricard, has 12 operating malt distilleries, a grain distillery, two gin distilleries and over 300 bonded warehouses in the UK, containing an extensive aged inventory of more than six million casks. Chivas Brothers ...
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Alderley Analytical - Case Study
Name: Sally Hannam Job Title: Director of Science Company: Alderley Analytical Ltd Alderley Analytical Ltd is based at the Alderley Science Park Bioanalytics with a touch of Genius Alderley Analytical Ltd is a bioanalytical research organization who offer laboratory services to companies without the physical resource or in-house expertise to carry out research within their own ...
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The Advantages of a Targeted 2D LC Approach
Webinar Highlights | Focus on the Relevant: The Advantages of a Targeted 2D Chromatography Approach Hi folks, I’m Daniel Root, Senior Product Marketing Manager for Multidimensional LC Systems at Waters. I recently conducted a webinar that addressed multidimensional liquid chromatography – an exceptional tool for the improvement of characterization and separation of highly complex and ...
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Oxidative transformation of oxcarbazepine by Cl2, ClO2 and O3: characteristics and pathways
In this research, the degradation efficiency, mechanism and intermediates’ toxicities of oxcarbazepine (OXC) upon chlorination, chlorine dioxide oxidation and ozonation were investigated. Results showed that three degradation approaches followed second-order kinetics, and ozonation had the highest removal efficiency both of OXC and dissolved organic carbon (DOC). Reaction intermediates were ...
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