assay Articles
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Cyst-Based Toxicity Tests. - XIV Application Of The Ostracod Solid Phase Microbiotest For Toxicity Monitoring Of River Sediments In Flanders (Belgium)
Abstract: In view of the need for rapid and low cost solid phase assays for contaminated sediments, a new 6 days “culture/maintenance free” microbiotest has been developed at the Laboratory for Biological Research in Aquatic Pollution at the Ghent University in Belgium, with the freshwater ostracod crustacean Heterocypris incongruens. The sensitivity of the Ostracodtoxkit FTM has ...
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Multiparametric comparison of chromogenic-based culture methods used to assess the microbiological quality of drinking water and the mFC method combined with a molecular confirmation procedure
MI agar and Colilert®, as well as mFC agar combined with an Escherichia coli-specific molecular assay (mFC + E. coli rtPCR), were compared in terms of their sensitivity, ease of use, time to result and affordability. The three methods yielded a positive E. coli signal for 11.5, 10.8, and 11.5% of the 968 well water samples tested, respectively. One hundred and thirty-six ...
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Genotoxicity testing of samples generated during UV/H2O2 treatment of surface water for the production of drinking water using the Ames test
in vitro and the Comet assay and the SCE testin vivo UV/H2O2 treatment can be part of the process converting surface water to drinking water, but would pose a potential problem when resulting in genotoxicity. This study investigates the genotoxicity of samples collected from the water treatment plant Andijk, applying UV/H2O2 treatment with an electrical energy dose of 0.54 kWh/m3 and a H2O2 dose of 6 mg/l. Genotoxicity was tested in vitro using ...
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Removal of norovirus from water by coagulation, flocculation and sedimentation processes
In this study, we determined the removal of a prototype human norovirus (Norwalk virus, NV) by bench-scale alum coagulation, flocculation and sedimentation processes using reverse transcriptase polymerase chain reaction (RT-PCR) for norovirus assays. After determining optimum conditions for the coagulation, flocculation and sedimentation processes in terms of turbidity reduction, jar tests were ...
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Cyst-Based Toxicity Tests. - XIII. Development Of A Short Chronic Sediment Toxicity Test With The Ostracod Crustacean Heterocypris incongruens. Methodology And Precision
Abstract: Experiments have been carried out with neonates of the freshwater ostracodHeterocypris incongruensto verify and complete former choices of test parameters for a new culture/maintenance free solid phase microbiotest for freshwater sediments. From the trials with increasing volumes of reference sediment, it was concluded that 300 µL was the most appropriate amount of sediment to be ...
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Effect-based trigger values for in vitro and in vivo bioassays performed on surface water extracts supporting the environmental quality standards (EQS) of the European Water Framework Directive
Abstract Effect-based methods including cell-based bioassays, reporter gene assays and whole-organism assays have been applied for decades in water quality monitoring and testing of enriched solid-phase extracts. There is no common EU-wide agreement on what level of bioassay response in water extracts is acceptable. At present, bioassay results are only benchmarked against each other but not ...
By QuoData GmbH
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Evaluation of the effects of varying the water velocity at the entrance of the contact zone on the performance of a dissolved air flotation (DAF) unit using the image analysis method
For a more efficient design of dissolved air flotation (DAF) units, it is important to deepen our knowledge of the role that each constituent part plays with regard to overall performance. The velocity control of the flocculated water at the entrance (Ve) of the contact zone of DAF units is important because the degree of agitation in this region may affect the size of the flocs and the ...
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Detection of
Pseudomonas aeruginosa by PCR in tap-water and bottled mineral water in the Isfahan province of IranThe purpose of this study was to consider the use of a simple polymerase chain reaction (PCR) as an accurate, safe and rapid method to detect Pseudomonas aeruginosa in tap-water and bottled mineral water from Isfahan province, Iran. A total of 224 tap-water and bottled mineral water samples were taken over six months, from July to December 2010. DNA (deoxyribonucleic acid) was extracted from ...
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Semi-quantitative estimation of Escherichia coli levels in public drinking water sources in northern Haiti
Prior research in Milot, Haiti, documented that public water sources are commonly fecally contaminated, as indicated by the presence of Escherichia coli. However, the degree of contamination was not assessed. In this study, the degree of fecal contamination in public drinking water sources was determined. Further, the usefulness of sanitary inspection surveys to predict fecal contamination ...
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Toxicity Determination of the Sediments of the River Seine in France by Application of a Battery of Microbiotests
Abstract : This study reports on the potential of the Toxkit microbiotests and the bacterial luminescence test Microtox®, to detect and quantify the toxicity of the sediments of the river Seine and a few tributary rivers in France. The objective of the research was to try to identify a minimum test battery for routine toxicity screening of river sediments. Attempts were also made to correlate ...
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Genotoxicity and effects of nanosilver contamination in drinking water disinfection
This study was conducted to examine the genotoxicity and the influence of silver nanoparticles (AgNPs) contamination when drinking water is exposed to five different disinfection treatments: chlorine, chlorine dioxide, ozone, ozone/chlorine and ozone/chlorine dioxide. Experiments were conducted with water samples of different chemical composition, from three water supply systems in Croatia. ...
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Washington: safe for humans?
Many stakeholders question whether EPA will carefully and consistently qualify what the test results mean – and do not mean. Hundreds of U.S. businesses have already received in the mail test orders issued by the U.S. Environmental Protection Agency (EPA) for initial endocrine screening under the Endocrine Disruptor Screening Program (EDSP). Hundreds more will receive orders later this ...
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Realistic environmental mixtures of micropollutants in surface, drinking and recycled water: Herbicides dominate the mixture toxicity towards algae
Mixture toxicity studies with herbicides have focused on a few priority components that are most likely to cause environmental impacts and experimental mixtures were often designed as equipotent mixtures; however, real world mixtures are made up of chemicals with different modes of toxic action at arbitrary concentration ratios. The toxicological significance of environmentally realistic ...
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Tecta B16 Success Story - Philippines Department of Health National Reference Laboratory - Case Study
The National Reference Laboratory (NRL) is the technical arm of the Department of Health (DOH) under the administration of the East Avenue Medical Center, a DOH hospital. A lab committed to excellence; NRL- EAMC is the Philippines’ reference laboratory for environmental, occupational health, toxicology and micronutrient assay. It functions to provide laboratory reference/referral ...
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Biological Water Testing
Improving Water Monitoring for Pathogens and other Microorganisms Water quality and purity is essential for public and environmental health but monitoring drinking water and wastewater for microbial orgamisms can be difficult and time consuming. In this article we want to highlight the Real-time PCR-based detection methods for some major pathogens and microorganisms that increases analysis ...
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IAQ investigators often overlook Bacteria as the Root Cause of Occupant Complaints
Untitled Document Many investigators have been dealing with mold issues the last few years and may have been overlooking bacteria as the underlying microbial problem. Bacteria typically outnumber fungi in the indoor air and may also account for foul odor problems. Bacterial contamination of HVAC systems has also been linked to hypersensitivity pneumonitis in building occupants. ...
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