Bioremediation Consulting, Incorporated

Low-pH tolerant Dehalococcoides (Dhc) Culture Developed by Bioremediation Consulting Inc


March 2011 -- BCI offers low-pH tolerant Dehalococcoides (Dhc) Culture

(March 2, 2011).  Bioremediation Consulting, Inc (BCI) of Watertown, MA announced today that it has completed development of a culture of low-pH tolerant Dehalococcoides (Dhc) bacteria to treat groundwater contaminated with chlorinated solvents such as perchloroethylene (PCE) and trichloroethylene (TCE) converting them to the harmless end product, ethylene (ethene) at pH as low as 5.7.

BCI’s cultures dechlorinate PCE and TCE at much more acidic groundwater conditions than can be tolerated by other commercial cultures of Dhc.  This breakthrough addresses one of the most pervasive and persistent problems in applying bioremediation to cleaning up groundwater contaminated with chlorinated solvents.  That problem is acidification of the groundwater during treatment and is usually caused by addition of excessive amounts of electron donor.  Using an acid-tolerant bacterial culture can be a cost effective alternative to applying buffering compounds to adjust the pH of entire aquifers.

 This project was funded by the National Science Foundation through a Small Business Innovation Research (SBIR) grant awarded to BCI in 2010.  BCI has been involved in cutting-edge research in the field of bioremediation for over 20 years and continues to advance the frontiers of this technology.

 This new culture has been added to BCI’s catalog of Designer BacteriaÔ (DB) and provide powerful new tools for many clients.  In addition to TCE, BCI has developed a wide range of Designer BacteriaÔto treat chlorinated benzenes, ethenes, ethanes and PCBs.   BCI’s Designer BacteriaÔ are grown in client’s ground water prior to field inoculation, to address specific site requirements, and to insure success of in situ bioremediation.

The new low-pH tolerant culture is now available for commercial clients.  Contact Sam Fogel for more information at


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