The manufacture and use of nanomaterials is increasing dramatically but there are serious concerns about the impact they may have on health and the environment.
One specific area of concern is how nanomaterials interact with cellular DNA perhaps causing damage and mutation. The Comet assay is one of several genotoxicity assays being used to measure nanoparticle induced DNA damage.
An increasing number of research groups conducting comet assays for this application use our Comet Assay scoring systems (http://www.perceptive.co.uk/cometassay/). The following peer reviewed articles have recently been published.
Gene toxicity studies on titanium dioxide and zinc oxide nanomaterials used for UV-protection in cosmetic formulations
(2010) R. Landsiedel et al, Nanotox. 4(4):364-381
In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: cyto-, genotoxicity and oxidative stress
(2010) S. Barillet et al, Toxicol. Letters 198(3):324-330
Thresholds for indirect DNA damage across cellular barriers for orthopaedic biomaterials
(2010) M Parry et al, Biomaterials 31(16):4477-4483
Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells
(2009) K. Gerloff et al, Nanotoxicology 3(4):355-364
Delphinidin Modulates the DNA-Damaging Properties of Topoisomerase II Poisons
(2009) M. Esselen et al, Chem. Res. Toxicol. 22(3):554-564
Genotoxicity evaluation of locally produced dental porcelain – An in vitro study using the Ames and Comet assays
(2009) M. Noushad et al., Toxicol In Vitro. 23(6):1145-50
Nanoparticles can cause DNA damage across a cellular barrier
(2009) G. Bhabra et al., Nature Nanotech. 4(1): 876-883
SiC nanoparticles cyto-and genotoxicity to Hep-G2 cells
(2009) S.Barillet et al J. Phys.: Conf Ser. 170 012016
To view these papers please visit our Recent Papers page (http://www.scorecomets.com/assay/#papers).