New certified reference material for genetically modified 98140 Maize
A new series of genetically modified organism (GMO) matrix reference materials has now been released. It contains four certified reference materials with different concentrations of 98140 maize (ERM-BF427). The CRMs are certified for their mass fraction of 98140 maize and are intended to be used for the quality control of quantitative measurements.
98140 maize carries the unique identifier DP-98140-6 and is engineered to express two new genes: the gat4621 gene isolated from Bacillus licheniformis that confers tolerance to glyphosate herbicide and the Zea mays hra gene produced by modifying the Zea mays als gene that provides tolerance to acetolactate synthase-inhibiting herbicides.
The four reference materials were prepared by mixing gravimetrically GM and non-GM maize seed powders. The resulting GM percentages were confirmed by real-time polymerase chain reaction (PCR) analysis after DNA extraction from the powders. The preparation and certification of ERM-BF427a, ERM-BF427b, ERM-BF427c and ERM-BF427d are described in detail in the certification report.
Legislation in the European Union requires labelling of food and feed products containing more than 0.9 % GMOs, provided the GMO has been placed on the market in accordance with Community legislation. Implementing the legislation requires suitable quality assurance tools, and laboratories need certified reference materials to be in accordance with lab accreditation schemes.
The materials have been processed and certified according to the ISO Guides 34 and 35.
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