Algae bloom is the result of a rapid accumulation of cyanobacteria in freshwater and other ecosystems.Water analysis brings a wide range of analytical challenges, especially during sample preparation. Waters` ACQUITY UPLC System with 2D Technologies employs solvent managers, a sample manager, a column manager, optical devices, the Xevo TQD mass spectrometer, and separation chemistries to facilitate multi-dimensional analyses. In this application note, a rapid, sequential micro-extraction protocol was evaluated for the analysis of microcystin RR, LR, and YR in bottled, tap, and surface water.