From ACE HILIC
Excellent peak shape and reproducibility have established ACE HPLC columns as the finest available. This quality is now available for protein chemists desiring the utmost in performance and reproducibility for the separation of peptides, proteins and other high molecular weight biomolecules. ACE 300Å columns are available in an extensive range of dimensions and particle sizes for use in micro-scale separations, LC/MS analyses, high speed preparative analyses up to process scale.
- Highly consistent silica surface providing excellent batch to batch reproducibility
- Available in high performing HPLC (3um and 5um) and UHPLC (1.7um) particle sizes
- Wide variety of column lengths and internal diameters available
- Hydrophilic Interaction Liquid Chromatography (HILIC) was first described by Alpert*
- HILIC is ideal for the separation and retention of polar species including polar neutral and polar ionised analytes
- HILIC separations typically include a polar stationary phase with high organic solvent containing mobile phases
- Mechanistically HILIC is complex (see Fig) and provides multiple modes of interaction between the analyte, stationary phase, eluent and water enriched layer at the stationary phase particle-eluent interface.
* A. J. Alpert, J. Chromatogr., 499 (1990) 177.
- HILIC provides the retention and separation of hydrophilic or polar to very polar analytes not well retained in RPLC
- Hydrophilic or polar to very polar analytes have log P values (measure of lipophilicity) of around zero or less (Fig 2A)
- Generally, polar analytes are suitable for HILIC if they elute before caffeine in gradient RPLC (Fig 2B)