From ACE HILIC
The ACE HILIC-N column is a bonded neutral character phase. The HILIC-N stationary phase is based upon the ultra-pure ACE silica platform with a proprietary polyhydroxy bonded functionality.
- Highly consistent bonded phase providing excellent batch to batch reproducibility
- Available in high performing HPLC (3um and 5um) and UHPLC (1.7um) particle sizes
- Wide variety of column lengths and internal diameters available
- Hydrophilic Interaction Liquid Chromatography (HILIC) was first described by Alpert*
- HILIC is ideal for the separation and retention of polar species including polar neutral and polar ionised analytes
- HILIC separations typically include a polar stationary phase with high organic solvent containing mobile phases
- Mechanistically HILIC is complex (see Fig) and provides multiple modes of interaction between the analyte, stationary phase, eluent and water enriched layer at the stationary phase particle-eluent interface.
* A. J. Alpert, J. Chromatogr., 499 (1990) 177.
- HILIC provides the retention and separation of hydrophilic or polar to very polar analytes not well retained in RPLC
- Hydrophilic or polar to very polar analytes have log P values (measure of lipophilicity) of around zero or less (Fig 2A)
- Generally, polar analytes are suitable for HILIC if they elute before caffeine in gradient RPLC (Fig 2B)