Amoeba

- Biological Biocid

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The only environmentally friendly solution effective against bacteria and biofilms. Amoéba's biocide has been extensively validated . First, it was tested in laboratory conditions and on biofilms using culture methods and molecular biology approaches.  Then, it went through 8 years of cumulated field applications in cooling towers (TP11). The main conclusions on its performance are presented here.

How does it work?    

  1. Amoeba's product, BIOMEBA, is a bacteriophage amoeba derived from a natural source.
  2. A natural predator of Legionella, pathogenic bacteria and free-living amoeba.
  3. Effective in the water phase and on biofilms.
  4. Ensures a level of Legionella below the current standards, both in the water phase and in biofilms.
  5. Operating conditions: Temperature between 53.6°F and 125.6°F; pH, from 5 to 10.
  6. Compatible with commonly-used anti-scaling and anti-corrosion products.
  7. Product not labelled with hazard warnings.   

Simplified implementation   

  1. Replacement of the previous chemical dosing system by a ready to use dosing cabinet for product injection, refilled easily thanks to quick connection system.
  2. Injection by dosing pump.
  3. Multiple injection strategies: continuous or shock dosage.   

Better than chemical solutions. Why?   

  1. Greater efficacy than current chemical alternatives.   
  2. Control of biological growth in circuits.   
  3. No need to pursue any multi-products program combining oxidising biocides, non-oxidising   
  4. biocides and biodispersants.   
  5. Stability of the product in the system.   
  6. 10 to 100 times lower volume of biocide necessary, depending on the dosing program.   
  7. The installation lasts longer.   
  8. Reduced need for blow-down, leading to water savings and cost reductions, as well as   
  9. contributing to sustainable development.   
  10. Reduced transportation costs and optimized carbon footprint.   
  11. Storage area reduced and secured compared to chemical biocides.   
  12. No personal protective equipment required.   

Additional benefits   

  1. Carton package 100% recyclable.
  2. Carton package easy to handle.
  3. The installation can be reduced to a simple dosing cabinet.

Rapid biocidal effect

In only a few hours, Green Biocide (BIOMEBA) kills microorganisms such as bacteria and amoebas, even encysted forms. It is highly effective against pathogenic bacteria included in a biofilm.

Broad-spectrum activity

Green biocide (BIOMEBA) is high efficient against planktonic and sessile bacteria ( legionella*, pseudomonas*, listeria*, Aeromonas hydrophila, klebsiella pneumoniae, E-coli, staphylococcus aureus, etc.) and onamoebas ( Acanthamoeba*, Hartmannella* and Naegleria Fowleri*), providing the highest safety levels for industrial installations.

Effective at low concentrations

No need to use a massive dose. 1 liter @ 3% treats 10 m3 of water; 1 liter @ 30% treats 100 m3.

Effectively eliminates biofilms

Amoéba's biological biocide naturally “grazes” on the biofilm. This was confirmed during the validation process using a highly realistic biofilm. Reduction of the biofilm by two orders of magnitude (100-fold) was also confirmed during field tests, showing that the “grazing” property is conserved in your water circuits (Total Viable Count and legionella count).

Effective over a wide range of pHs and temperatures

Green biocide was shown to be effective at pHs ranging from 5 to 10.
Its efficiency was also validated at temperatures from 12°C to 52°C (EN 13623 regulation and other regulations).

No environmental concerns (biodegradable and non-persistent in the environment)

Amoéba's biological biocide is 100% biodegradable. Since it consumes microorganisms, it does not produce any residues, and it disappears naturally 4 days after the last injection. As there is no need for deactivation, you can already meet the regulations relating to waste-water management that will soon be applied! More generally, your installation will conform with sustainable development rules.

Compatible with chemical or organic compounds commonly found in water circuits

Green biocide is compatible with commercial anti-scaling agents, anti-corrosion agents and anti-algae products, both at normally used concentrations, and at massive doses.

Amoéba’s product has also been proven to be tolerant to other contaminants, such as oil.

Infrequent dosing

Green Biocide persists in the system for a few days. Because it sticks to the biofilm, Amoéba's biocide ensures continued action between two injections, and blow down does not completely eliminate it from the system.

Planktonic and sessile bacteria

The minimum killing effect of our Green biocide/BIOMEBA was tested as for antibiogram testing. Results showed a phagocytotic plaque after addition of our biological biocide, effective against all the bacterial strains in the following table. In addition, Green biocide/BIOMEBA was demonstrated to perform better than all chemical biocides during a 2-year field test period in several companies, with daily monitoring of its effect on Legionella.

Amoeba (replicative forms and encysted forms)

Amoéba's Green biocide/BIOMEBA has been confirmed to be effective against the following amoebas, whether they are in replicative or cystic forms.

Realistic biofilms

In just 24 hours, a single application of our biological biocide can decrease the total viable count on a mature biofilm composed of 4 bacteria - including Legionella pneumophila - by 99%.

Moreover, a single dose prevents the reformation of the biofilm over the following 4 days.

Amoéba wished to test the limits of efficacy for its Green biocide/BIOMEBA in this application, since it is generally recognised that 95% of pathogenic bacteria are included in biofilms. Because of this observation, an effective biocide must kill sessile pathogenic bacteria present in the biofilm, but it must also reduce the total viable count in highly realistic biofilms.

Amoéba has been extremely thorough in validating its biocide's activity against biofilms. Our researchers, in collaboration with worldwide biofilm specialists, have designed a highly realistic mature biofilm, composed of at least 4 bacteria and amoeba.

Mature biofilm is formed by incubation and growth for more than 7 days in culture media. In contrast, an early biofilm consists of a pre-grown biofilm less than 48-hours-old.

The inclusion of multiple bacteria is mandatory because the interaction between the various bacterial strains leads to an inter-bacterial mechanism of resistance.

The presence of amoebas was also tested, since amoebas play a key role in the acquisition of bacterial resistance to chemical biocides. (1 ppm of CMIT/MIT is required to inhibit the development of free legionella; whereas 16 ppm of CMIT/MIT is required to inhibit the same legionella concentration after pre-incubation with amoebas).

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