The ANDRITZ high-gradient magnetic separator allows the extraction of one protein fraction directly from the non-purified complex feedstock. With HGMS technology, the efforts of downstream processing can be decreased drastically while the yield is increased.
Various studies have been conducted in high-gradient magnetic separation (HGMS) technology over the past few years. The major advantages of this technology are obvious when processing highly complex feedstocks, such as blood. The current state-of-the-art technology for protein fractionation is liquid chromatography, which needs several time-consuming and costly upstream purification steps to be able to process the feedstock. In contrast, HGMS allows the extraction of one protein fraction directly from the non-purified complex feedstock. With HGMS technology, the efforts of downstream processing can be decreased drastically, while the yield is increased.
- High specific and selective protein harvesting
- Separation efficiency of target protein up to 95% (depending on reaction time and magnetic particle concentration)
- Replacement of purification steps, cell separation and chromatographic fractionation
- Extreme reduction of separation time down to several minutes
- Increased yield with less effort
- Reduced down streaming efforts to reach final pureness
- Magnetic particles
The principle of the technology is to bind a specific protein fraction to magnetic beads with a highly selective functionalized surface. By using a magnetic field, it is possible to extract the magnetic beads and also the specific fraction from a non-purified feedstock together with these beads in a single unit operation. Application fields for this technology are slurries involving high downstream effort, extremely low titer, and highly valuable components like hormones, antibodies, enzymes, or simply the functionalized particles themselves.
Research was conducted on the magnetic beads, the selective binding, and the elution process. The first rotor-stator magnetic separator systems were built in order to evaluate the separation principle. The separation, particle life time as well as elution processes were evaluated and proven.
To take the HGMS to the next stage, a new design concept was developed for the rotor-stator technology, a quantum leap in terms of yield and harvesting efficiency as well as cleaning and sterilization. The ANDRITZ high-gradient magnetic separator design is complaint to:
- ASME BPE
Therefore the units can be applied both for research and for GMP production.