AquatiqPCR - C/G Molecular Detection of Pathogenic Cryptosporidium and Giardia in Water
Advantages: No need for an expert eye – Dismiss microscopic examination. Fast – Definitive results are achieved in a matter of hours. Sensitive – Less than 5 oocysts/cysts per sample (filtrate of 50l). Specific – Prevalent pathogenic species only*. Robust – Excellent reproducibility on any qPCR instrument . Cost effective. A lab can process tens to hundreds samples a day as opposed to today’s routine of only a few per day. Low TCO (Total Cost of Ownership) is a fraction of the cost of current tests. Decreases manpower costs – Can be operated by any molecular biologist.
AquatiqPCR©-C/G** 30-001 (24 samples) Multiplex, qPCR water testing kit for the simultaneous detection of pathogenic Cryptosporidium and Giardia in the same tube. AquatiqPCR©-C** 30-002 (24 samples) Singleplex qPCR detection of pathogenic Cryptosporidium AquatiqPCR©-G** 30-003 (24 samples) Singleplex qPCR detection of pathogenic Giardia * For research use only ** C. parvum and C. hominis and G. lamblia assemblages A&B. Assay specificity The assay is specific for pathogenic Cryptosporidium species and Giardia assemblages A&B. Other related protozoa that were tested (e.g., Entamoeba histolitica, Babesia canis, Babesia gibsoni, Theileria annae, Toxoplasma gondii and Trypanosoma brucei) showed negative results under the same conditions.
Quantification The above is a typical standard plot of serial decimal dilutions of target Cryptosporidium and Giardia DNA sequences (green dots). Blue dots indicate water samples containing 30 and 3 Cysts/Oocysts, respectively. Environmental water samples were spiked with Cysts and Oocysts from validated spiking stocks (Waterborne, Inc.) and tested with AquatiqPCR©-C/G kit. Results The number of Giardia Cysts and Cryptosporidium Oocytes can be deduced by a standard plot based on serial decimal dilutions of target DNA oligo’s. Taking into account that every Giardia Cyst contains 8 sets of genomic DNA and each Cryptosporidium Oocyst contains 4 sets, we are able to translate the DNA copy number from the qPCR reaction into actual numbers of Cyst/Oocyte.