Aboatox Oy

- Model 1243-500 - Bio Toxkit

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The BioTox Kit is used for the determination of toxicity of water soluble samples. The inhibitory effect of the sample on the
light emission of luminescent bacteria, Aliivibrio fischeri (formerly Vibrio fischeri), is measured with a luminometer. The
BioTox Kit provides a rapid, easy to use method for measuring toxicity of aqueous samples such as WASTE WATER,
AQUEOUS EXTRACTS AND LEACHATES, FRESH WATER, SEA AND BRACKISH WATER, ELUATES OF SEDIMENT, PORE WATER AND SINGLE SUBSTANCES. SOIL AND SEDIMENT samples can also be tested with the kinetic modification of the test

ASSAY PRINCIPLE
The inhibition of the luminescence is determined by combining different dilutions of the test sample with luminescent bacteria.
The decrease of light intensity is measured after a contact time of 5 - 30 minutes. The inhibitory effect of dilutions is compared
to a toxin free control to give the percentage inhibition (INH%). The value is plotted against the dilution factor and the resultant
curve is used to calculate the EC 50 (Effective Concentration causing 50% inhibition of light output) of the sample.

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