- Model 205 - Capillary Electrophoresis System

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Capel-205 is a new generation capillary electrophoresis system with a significantly extended autosampler for continuous running of dozens of analyses and increased efficiency in separation.

Capel-205 is a new generation capillary electrophoresis system with advanced options and design. Its main features are:

  • Extended autosampler capacity up to 59 positions, allowing to provide overnight analyses
  • Standard centrifuge-type vials of 1.5mL volume (Eppendorf-type) closed with their original caps are used for all solutions
  • Specially designed unit automatically opens vials just prior to their use, thus preventing evaporation and any kinds of cross-contamination
  • New HV block provides voltage up to +/- 30 kV thus improving separation efficiency and decreasing analysis time
  • Optionally – integrated unit to control the state of the inner capillary surface provides significantly improved migration time reproducibility especially when analyzing samples with complex matrices

Thus Capel-205 can be the very best choice not only for R&D and low-scale analyses but also for the customers dealing routinely with dozens of samples per day.

High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance.

Extended instrumental options

  • Extended autosampler capacity up to 59 positions – hundreds of samples per months can be analyzed
  • Integrated vials caps removal – all solutions are kept in the autosampler tightly closed excluding evaporation and cross-contamination
  • New HV block provides voltage up to +/- 30 kV – separation efficiency is improved and analysis time is decreased
  • Redesigned capillary cassette – length of the thermostated part of the capillary is increased to improve migration time reproducibility
  • Spectra scanning facilitates peak identification
  • Broad range of controlled injection pressure allows analysis of viscous samples
  • Reverse sample injection under vacuum: ultra-short analysis time (less than 1 min) and sample stacking to decrease detection limit

Precise and highly effective capillary thermostating provided by circulating liquid coolant (water)(± 0.1 C)

  • Extended range of applied buffers and increased efficiency in separation

Powerful software package 'Elforun'

  • Modified Elforun-205 to become even more user-friendly when operating with extended autosampler capacity
  • Increased flexibility in performing analyses of various complexity
  • Any kinds of complex runs are possible including those with pre-programming of changes in analysis conditions
  • Customized report, data export to other programs

Unique design of the capillary cassettes

  • Enlarged thermostatic part of the capillary improves migration time reproducibility
  • Easy capillary change in just a few minutes
  • Lower Detection Limit due to the optimized optical scheme

Detectors

  • CAPEL-205 is equipped with a spectrophotometric detector with a wavelength range of 190 - 400 nm.

Light source

  • Deuterium lamp

Vials

  • Standard centrifuge-type (e.g.Eppendorf-type)1.5 ml, minimal sample volume 100 µl.

Injection modes

  • Sample injections by pressure, vacuum or electrokinetically.
  • Controlled pressure profile of injection with pressure values from – 100 mbar to + 100 mbar.
  • Electrokinetic injection at (+/-) 1 – 30 kV considering time for voltage ramping.
  • Programmable injection time / pressure / voltage.

Analysis

  • With a Voltage from -30 kV to +30 kV with a 1 kV step and an electronic polarity switch.
  • Current from 0 to 300 µA.

Elforun software features

  • Combination of instrument controlling, data acquisition and data processing;
  • Real time electropherogram visualization;
  • Electropherogram data processing with overlay mode and basic mathematical operations;
  • Computation of electrophoresis system parameters;
  • Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors;
  • Extensive data processing algorithm customization;
  • GLP and GALP compliance.

  • Capillary electrophoresis system CAPEL-205;
  • Spare capillary cassettes;
  • Elforun software package;
  • Kits for analyses (by request); most of CE-kits of any manufacturers are compatible with Capel-205.

Warranty

  • All Lumex instruments and equipment are covered by a 12-months warranty.

Installation of LUMEX instruments can be carried out at a Customer's site by our service engineers. Personnel training specific to the Customer needs can be also provided.

  • Detector: Spectrophotometer 190-400 nm
  • Analysis:
    • Constant voltage (+/-) 1 - 30 kV in 1 kV steps
    • Automatic (electronic) polarity switch
    • Current 0 - 300 mkA
    • Pressure (+/-) 1-100 mbar
    • Programmable changing of wavelength, pressure and voltage during analysis
  • Injection:
    • By voltage
    • By pressure
    • By vacuum
  • Sampler: Autosamplers for 59 vials
  • Rinsing: With pressure ~1000 or 2000 mbar
  • Capillary:
    • Length: 30-100 cm
    • Internal diameter: 50, 75, 100 μm
  • Capillary thermostating: Liquid thermostating conducted at temperatures from 10 ºC below to 30ºC above ambient temperature (± 0.1 C)
  • Controlled parameters:
    • Wavelength
    • Pressure
    • Temperature
    • Voltage
  • Power requirements: 110/220 Vac, 50/60 Hz
  • Power consumption: 170 W
  • Size, LxWxH, cm: 470х530х410
  • Weight, kg: 30

  • Environmental analysis: water and soil quality monitoring (cations, anions, herbicides, pesticides);
  • Food industry: analysis of beverages and juices (cations, anions, vitamins, organic acids, preservatives, antioxidants, dyes, sweeteners); mineral water (cations, anions); wines and brandies (cations, organic acids, polyphenols, aldehydes, sulfite); beer (inorganic cations and anions, hop and bitter acids, sulfite, amino acids, organic acids, vitamins); milk (proteins, antibiotics); tea, coffee (caffeine, polyphenols); foodstuff (amino acids, synthetic dyes, organic acids, amines, proteins, melamine);
  • Chemical industry: technological monitoring, analysis of raw material;
  • Pharmaceutical industry: patent medicines analysis, technological monitoring, and enantiomers separation;
  • Biochemistry: analysis of proteins by capillary isoelectric focusing technique, pharmacokinetics studies, hemoglobin variants determination;
  • Forensic studies: analysis of explosives and trace detection, drugs analysis, analysis of writing paper components.

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