Chromaflow - Chromatography Columns
From Chromatography Products - Columns - Process columns
A family of convenient process-scale columns with a patented nozzle in the top and bottom of the column that allows packing, unpacking, and cleaning in place. Chromaflow columns are a natural choice when scaling up from BPG columns.
Overview
A family of convenient process-scale columns with a patented nozzle in the top and bottom of the column that allows packing, unpacking, and cleaning in place. Chromaflow columns are a natural choice when scaling up from BPG columns.
- For capture or intermediate purification.
- Scalable up to 2 m in diameter.
- Easy to operate and maintain. Packing knowledge and application support available.
- Convenient, pack-in-place nozzle.
- Closed system improves safety.
- Easy-to-use Chromaflow packing station facilitates packing procedure.
- All plastomers in contact with process flow are tested and approved to USP Class VI.
- All metallic materials in construction are fully traceable.
- Installation and Operational Qualification Documentation Packages and service and maintenance contacts available.
Do it all without even lifting the lid
With Chromaflow columns, packing, operation, unpacking, and cleaning can be performed without removing the lid or adapter – all due to the design of the nozzle in both the top and bottom end-pieces. With the establishment of packing protocols for individual resins, large-scale chromatography is more convenient, scalable and safer for both the operators and the product. Chromaflow columns are intended for GMP production.
How it works
Nozzles sit in both the top and bottom of the column allowing packing in either an upward or downward direction. There are three nozzle positions:
- Mid-position, for priming and packing. The bottom nozzle extends part of the way (mid-position) into the column. The top nozzle is fully retracted. Slurry enters the column via the bottom nozzle and excess liquid exits via the top mobile phase outlet. After packing, the slurry lines are isolated from the mobile phase and can be cleaned independently from the rest of the column.
- Retracted, for running. The top and bottom nozzles are retracted. Mobile phase enters the column directly into an annulus immediately behind the bed support. The annulus is cut through at an angle to ensure that the linear flow rate is kept constant during distribution of the mobile phase across the bed.
- Fully extended into the column for unpacking and cleaning. In this position, both bottom and top nozzles are fully extended into the column, thereby exposing a third passage through which resin leaves the column. Cleaning solution can be pumped through the nozzles and sprayed into the column. In this way, the column is easily and effectively cleaned without exposing the interior or the resin to the outside, or without dismantling the column.
The nozzle also enables isolation of slurry lines from the mobile phase during operation, allowing removal of residual resin and cleaning of the slurry lines independently of the rest of the column.
Product Specifications
- Parameter: Chromaflow 400 mm, SFP manual nozzle, 200-400 mm, 10 SS
- Adapter Movement: Manual
- Bed support: Net
- Bed Volume Max.: 51 l
- Bed Volume Min.: 26 l
- Column Cross Section Area: 1260 mm2
- Compliance: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva and connected only to other CE labeled Cytiva modules or other products as recommended. For regulatory details please see declaration of conformity.
- Connections: 25 mm TC
- Footprint: 700 x 700 mm
- Inlet Dimension i.d.: 14 mm
- Material (column tube): Acrylic PMMA
- Material (Distributor): Polypropylene (PP)
- Material (Lid): 316L Stainless Steel (SS)
- Material (Nozzle Body): Polypropylene (PP)
- Material (Nozzle Tip): Polyetheretherketone (PEEK) 450G
- Material (Nozzle Tube): 316L
- Material (Seal): Ethylene Propylene Diene (EPDM) and FEP Encapsulated Silicone
- Material (Stand): 316 Stainless Steel (SS)
- Operating pressure max.: 3 bar (43.51 psi)
- Operating Temperature: 4°C-30°C
- Order Information: For diameters between 1200 and 2000 mm, please inquire with your Cytiva representative.
- Outer Height: 1568 mm
- Surface Finish, Stainless Steel Internal Wetted: Ra Electropolished'< 0.5 µm
- Surface Finish Stainless Steel Non-Wetted Parts: Ra < 3 µm
- Weight: 230 kg
- Column i.d.: 400 mm
- Porosity: 10 µm
- Material [Bed Support]: 316L and S32205 Stainless Steel (SS)
- Nozzle Type: SFP Manual nozzle
- Bed Height: 200-400 mm
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