Modern Water - Monitoring Division

Modern Water - Monitoring Division

- Rapid Field Enzyme Immunoassay

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EnviroGard is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and/or water for remediation, assessment, and industrial testing. It uses an antibody coated test tube or plate technology to detect the analyte of concern. The method can be used to test for analytes such as pesticides, Microcystins and PCBs.

EnviroGard test kits allow for quick turnaround time on results while also offering highly accurate data for remedial investigation, Microcystin detection, and pesticide and herbicide detection.

The EnviroGard tube based methods are completely field portable and generate on-site data giving way not just to a time efficient process, but a cost efficient one as well.

The EnviroGard plate kits are fully quantitative, laboratory based tests that generate results with high precision.

In addition to soil and water matrices, the EnviroGard PCB test can be used for wipe samples and testing for PCBs in concrete and wood.

  • Quantitative or semiquantitative results
  • Test up to 17 samples at once
  • Results available in 30-90 minutes
  • Many methods associated with an SW-846 method

The EnviroGard kits apply the principles of enzyme linked immunosorbent assay (ELISA) to the determination of the target analyte and related compounds.

The sample and an enzyme conjugate are added to a test tube or plate well that is coated with antibodies specific to the target analyte. Both the target analyte and the enzyme labelled analyte compete for antibody binding sites on the immobilised antibodies. The analyte and labelled analyte analog are bound to the antibodies on the wall of the tube or plate well in proportion to their original concentration.

After decanting and washing unbound reagents from the tube or plate well, a chromogenic substrate is added. The enzyme labelled analyte analog bound to the antibody catalyses the conversion of the chromogenic substrate to a coloured product and after an incubation period is stopped and stabilised using an acid.

Because the labelled analyte (conjugate) was in competition with the unlabelled analyte (sample) for the antibody sites, the colour that develops is inversely proportional to the concentration of analyte in the sample. In other words, the darker the colour, the lower the concentration in your sample.

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