Analytik Jena AG

- Blood DNA Midi Kit



The innuPREP Blood DNA MIidi Kit has been specially designed as an effective tool for isolating genomic DNA from 0.5 ml to 2 ml samples of whole blood. The kit combines three steps: erythrocytes are first selectively digested, after which nucleated blood cells are pelleted and then lysed. After a precipitation step to selectively remove proteins, the DNA is bound to the surface of a Spin Filter membrane and then eluted. The extraction process involves the use of standard bench-top centrifuges and operates on a minicolumn scale. Combining a highly stringent Lysis Buffer with a novel Binding Buffer makes it possible to achieve unique yields of highly pure DNA.

  • Extremely simple tool for isolating genomic DNA from 0.5 ml to 2 ml whole blood samples
  • Excellent purity and yields of up to 50 µg
  • Processing in mini-Spin Filter format despite large starting volumes
  • Patented, optimized extraction chemistry

Starting material:

  • 0.5 to 2 ml samples of whole blood
  • Fresh or frozen blood
  • Stabilizers: EDTA or citrate

Extraction time:

Approx. 30 – 40 minutes, including lysis

Binding capacity:

Column binding capacity: > 100 µg gDNA

Average yield:

  • Depends on the sample and the volumes used
  • Approx. 10 – 50 µg gDNA

Average purity:

1.7 – 2.0

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