Analytik Jena  - an  Endress+Hauser Company

- Blood RNA Kit

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The innuPREP Blood RNA Kit allows users to extract total cellular RNA from fresh or frozen whole blood samples, which were stabilized with either EDTA or citrate. The specially optimized Lysis Buffer makes lysis extremely efficient, effectively deactivating endogenous and exogenous RNases. Using innuPREP Blood RNA Kit allows researchers to isolate highly pure RNA in an extremely short amount of time while completely omitting the highly toxic β-mercaptoethanol traditionally used in the extraction process. Upon elution in RNase-free water, the final RNA is ready-to-use and can be integrated in subsequent applications immediately.

  • Isolation of total cellular RNA from whole blood samples of up to 1 ml
  • Selective removal of genomic DNA with no DNase digestion
  • Efficient inactivation of endogenous and exogenous RNases
  • Extraction of highly pure RNA with no degradation
  • No use of toxic β-mercaptoethanol

Starting material:

  • 0.5 – 1 ml whole blood samples
  • Fresh or frozen blood
  • Stabilizers: EDTA or citrate

Extraction time:

Approx. 45 minutes

Binding capacity:

Column binding capacity: > 20 µg RNA

Average yield:

  • Depends on sample
  • Approx. 1 –8 µg

Average purity:

1.8 – 2.1

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