Analytik Jena AG

- RNA Mini Kit 2.0

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The innuPREP RNA Mini Kit 2.0 is a jack-of-all-trades when it comes to extracting total RNA. The specially optimized Lysis Buffer system guarantees isolation of intact RNA and lasting deactivation of endogenous and exogenous RNases. A precolumn contained in the kit can be used to remove genomic DNA, which utterly eliminates the traditional need for DNase I digestion. The RNA is then bound to a second Spin Filter membrane, washed and finally eluted in 30 – 80 μl of RNase-free water. As such, the extraction process can be concluded in 15 – 40 minutes, depending on the starting material. Compared to the precursor version extraction protocols as well as Spin Filter columns are optimized and thus guarantees total RNA that is enhanced in terms of both quality and quantity.

  • Fast, efficient purification of total RNA
  • Applicable for a wide variety of different starting materials and in varying amounts
  • Kit for RNA isolation from tissues samples and biopsies, eukaryotic and bacterial cells (gram- and gram+)
  • Selectively removes genomic DNA with no DNase digestion
  • Eliminates need for using toxic β-mercaptoethanol
  • Excellent quality (A260:A280 and A230:A260) and high quantity of isolated RNA
  • Optimized protocols can be combined with innuSPEED Lysis Tubes

Starting material:

  • Eucaryotic cells (max. 5 × 106)
  • Tissue samples (max. 20 mg)
  • Gram+ and gram– bacteria (max. 1 × 109)
  • Biopsies

Extraction time:

Approx. 15 to 40 min

Quality:

1.8 – 2.1 (A260:A280)

Binding capacity:

Approx. 100 μg RNA

Average yield:

  • Abhängig von der Art und Menge des Ausgangsmaterials
  • Bis zu 100 μg RNA

Average purity:

1.8 – 2.1

Volume:

30 - 80 μl RNase-free water

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