Analytik Jena  - an  Endress+Hauser Company

- RNA Virus Kit-KFFLX

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The innuPREP RNA Virus Kit - KFFLX can be used to automate extraction of viral RNA from up to 96 samples in just approx. 45 minutes. The method, which is based on magnetic particle separation and on the use of patented extraction chemistry in the KingFisher® FLEX system, can be used for isolating an extremely wide variety of starting materials, such as cell-free bodily fluids, biopsies, swabs and stool samples. The isolated viral nucleic acids are then available for immediate use in downstream applications. The high quality of the RNA has been successfully tested and confirmed using the TaqMan® real-time PCR (after cDNA synthesis).

  • Optimized for isolating viral RNA under high-throughput conditions
  • Use of the KingFisher® FLEX automation system for running up to 96 samples in parallel
  • Automated elution in a 96 well plate
  • For a kit with extraction control, please use the innuPREP RNA Virus PLUS Kit-KFFLX

Starting material:

  • Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl)
  • Cell culture supernatant, enrichment medium (up to 200 µl)
  • Tissues (approx. 1 - 5 mg)
  • Swab samples
  • Stool samples (approx. 50 - 100 mg)

Extraction time:

  • Manual lysis: tissue samples only (30 – 60 minutes)
  • KingFisher® FLEX lysis protocol: approx. 20 minutes
  • KingFisher® FLEX protocol: approx. 37 minutes

Quality of viral RNA:

Tested with positive results in cDNA synthesis followed by TaqMan® real-time PCR

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