Analytik Jena  - an  Endress+Hauser Company

- Soil DNA Kit

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The innuSPEED Soil DNA Kit is a tool for extracting microbial DNA from soil samples. The extraction combines rapid homogenization of extremely complex starting material with efficient digestion of microbial cell walls in specially developed Lysis Tubes (using a SpeedMill or other homogenizer). After breaking down the material mechanically followed by a thermal lysis step, the DNA is selectively bound to the surface of a Spin Filter membrane. A novel wash buffe efficiently removes inhibitors such as humic acids. Finally, DNA is de sorbed from the membrane through the addition of a low-salt buffer.

  • Isolation of microbial DNA from soil samples
  • Includes homogenization (SpeedMill, for instance) using Lysis Tubes specific to the application 
  • High-quality DNA free of any inhibitors (such as humic acids)
  • Suitable for use with homogenizers (such as the SpeedMill
    or others)

Starting material:

Soil samples (100 – 250 mg)

Extraction time:

  • Homogenization and lysis: 30 minutes
  • Purification: approx. 16 minutes

Binding capacity:

Column binding capacity: > 30 µg DNA

Average yield:

  • Depends on the type and quantity of microorganisms in the soil
  • Up to 30 µg

Average purity:

1.7 – 2.0

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